Comment[ArrayExpressAccession]	E-GEOD-44069
MAGE-TAB Version	1.1							
Public Release Date	2013-04-01
Investigation Title	Altered expression of microRNAs in PC-3 sphere cells of prostate cancer compared with PC-3 adherent cells							
Comment[Submitted Name]	Altered expression of microRNAs in PC-3 sphere cells of prostate cancer compared with PC-3 adherent cells
Experiment Description	Metastatic prostate cancer is a leading cause of cancer-related death in men. Cancer stem cells (CSCs) are involved in tumor progression and metastasis, including in prostate cancer. There is an obvious and urgent need for effective cancer stem cells specific therapies in metastatic prostate cancer. MicroRNAs (miRNAs) are an important class of pervasive genes that are involved in a variety of biological functions, especially in cancer. The goal of this study was to identify miRNAs involved in prostate cancer metastasis and cancer stem cells. Several published reports have demonstrated that non-adherent spheres culture is increasingly used as an effective method to enrich and identify stem cells or putative CSCs.In our previous study, we enriched prostate cancer stem cells from PC-3 sphere cells in serum-free suspension culture and characterized their CSCs properties.Thus, we used spheres as a prostate cancer stem cells model to elucidate its metastatic mechanisms. We examined the miRNA expression profiles of PC-3 sphere cells of prostate cancer compared with PC-3 adherent cells by miRNA microarray.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Chen	Fan	Chen	Deng	Zhong	Cai	Lin	
Person First Name	Xu	Xinlan	Xu	Weixi	Guangzheng	Qingqing	Tianxin	
Person Email	joshua_18chen@163.com							
Person Affiliation	Sun Yat-sen Memorial Hospital							
Person Address	Department of Urology, Sun Yat-sen Memorial Hospital, 107. W. Yanjiang Road, Guangzhou, Guangdong, China							
Person Roles	submitter							
Protocol Name	P-GSE44069-1	P-GSE44069-6	P-GSE44069-3	P-GSE44069-8	P-GSE44069-7	P-GSE44069-2	P-GSE44069-4	P-GSE44069-5
Protocol Description	ID_REF =  VALUE = Normalized signal intensity	We followed the manufacturer's protocol of the mirVana(TM) miRNA bioarray.	The human prostate cancer cell PC-3 (ATCC, Manassas, VA) is cultured in RPMI-1640 medium (Gibco, Invitrogen) supplemented with 10 % FBS (Hyclone)	We used DMVS (Chipscreen Biosciences, Ltd.) for quantification. We performed global median normalization and probe summarization by average.	We followed the manufacturer's protocol, and used LuxScan TM 10K(CapitalBio, Ltd) to scan the microarray.The images of the hybridized arrays were processed with GenePix Pro 6.0 (Axon, Ltd) and signal intensities quantified	In order to enrich the prostate CSCs, PC-3 cells were grown to 90 % confluence, trypsinized, and plated at a density of 1,000 cells/ml in serum-free DMEM/F12 medium (Gibco, Invitrogen) containing 20 ng/ml epidermal growth factor (EGF, R and D Systems, MN), 5 μg/ml insulin, 0.4 % bovine serum albumin (Sigma, St. Louis, MO), and 2 % B27 (Invitrogen, CA) in 10 cm2 culture dishes.	We used the TRIzol Reagent (Life Technologies) and mirVana(TM) Isolation Kit following the manufacturer's protocol.	We used the mirVana(TM) miRNA Labeling kit (Ambion) following the manufacturer's protocol
Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
Experimental Factor Name	CANCER STEM CELLS PROPERTY	CULTURE						
Experimental Factor Type	cancer stem cells property	culture						
Comment[SecondaryAccession]	GSE44069							
Comment[GEOReleaseDate]	2013-04-01							
Comment[ArrayExpressSubmissionDate]	2013-02-05							
Comment[GEOLastUpdateDate]	2013-04-01							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-44069.sdrf.txt