Comment[ArrayExpressAccession] E-GEOD-44022 MAGE-TAB Version 1.1 Public Release Date 2013-12-22 Investigation Title Expression profiling of cisplatin resistant derivatives of ovarian cancer cell line A2780 Comment[Submitted Name] Expression profiling of cisplatin resistant derivatives of ovarian cancer cell line A2780 Experiment Description The goal of this study was to determine genes with altered expression after selection of ovarian cancer cells for survival in increasing concentrations of cisplatin. A series of progressively cispatin resistant derivatives of A2780 ovarian cancer cell lines was hybridized to spotted cDNA microarrays using two color technology using the parental A2780 cells as a reference. Duplicate hybridizations were carried out for each resistant derivative. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Zhu Meltzer Gonda Person First Name Yuelin Paul Tamas Person Mid Initials Jack Person Email zhujack@mail.nih.gov Person Affiliation NCI Person Phone (301)496-4527 Person Address Genetics Branch, Center for Cancer Research, NCI, 9000 Rockville Pike, Bethesda, MD, USA Person Roles submitter Protocol Name P-GSE44022-1 P-GSE44022-4 P-GSE44022-5 P-GSE44022-2 P-GSE44022-3 P-GSE44022-6 Protocol Description Data was extracted from the scans using Dearray software in IP Labs as described at http://research.nhgri.nih.gov/microarray/image_analysis.shtml ID_REF = VALUE = log2 normalized test/reference PRE_VALUE = normalized ratio Cy5/Cy3 RNA was labeled with Cy3 or Cy5 by reverse transcription in the presence of dye-coupled dUTP. Labelled cDNA was hybridized as described at http://research.nhgri.nih.gov/microarray/hybridization.shtml Normal growth conditions in RPMI1640 with 10% fetal calf serum. Total RNA extracted using Trizol following manufacturer's instructions Slides were scanned in an Agilent G2565AA microarray scanner. Protocol Type normalization data transformation protocol labelling protocol hybridization protocol growth protocol nucleic acid extraction protocol array scanning protocol Experimental Factor Name CISPLATIN REACTION CISPLATIN DERIVATIVE Experimental Factor Type cisplatin reaction cisplatin derivative Comment[SecondaryAccession] GSE44022 Comment[GEOReleaseDate] 2013-12-22 Comment[ArrayExpressSubmissionDate] 2013-02-01 Comment[GEOLastUpdateDate] 2013-12-22 Comment[AEExperimentType] transcription profiling by array Comment[AdditionalFile:Data1] GSE44022_A2780_cisplatin_raw_channel_data.txt SDRF File E-GEOD-44022.sdrf.txt