Comment[ArrayExpressAccession]	E-GEOD-43242
MAGE-TAB Version	1.1															
Public Release Date	2014-01-21
Investigation Title	Effect of osteoblast-specific constitutive activation of beta-catenin or deletion of FoxO1 on gene expression in mice															
Comment[Submitted Name]	Effect of osteoblast-specific constitutive activation of beta-catenin or deletion of FoxO1 on gene expression in mice
Experiment Description	The gene expression of mice with osteoblast-specific beta-catenin activation or FoxO1 deactivation are each compared to that of Wt. A. Wt mice (n=4) B. Osteoblast specific beta-catenin constitutively expressed mice (n=3) C. Osteoblast specific FoxO1 deleted mice  (n=3)															
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl														
Person Last Name	Kousteni	Kode	Manavalan	Mosialou	Rathinam	Bhagat	Vundavalli	Friedman	Pickard	DePinho	Galil	Raza	Mukherjee	Teruya-Feldstein	Berman	Kousteni
Person First Name	Stavroula	Aruna	Sanil	Ioanna	Chozha	Govind	Murty	Richard	David	Ronald	Naomi	Azra	Siddhartha	Julie	Ellin	Stavroula
Person Mid Initials			J		V			A		A						
Person Email	geo@ncbi.nlm.nih.gov															
Person Affiliation	Columbia University Medical Center															
Person Address	Medicine, Columbia University Medical Center, 1150 St. Nicholas Av, New York, NY, USA															
Person Roles	submitter															
Protocol Name	P-GSE43242-1	P-GSE43242-4	P-GSE43242-5	P-GSE43242-2	P-GSE43242-3	P-GSE43242-6										
Protocol Description	Data was normalized using the MAS5 algorithm ID_REF =  VALUE = Mas5 signal intensity log2 transformed	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).	12.5mg of aRNA was hybridized to Mouse Genome 430. 2.0 GeneChips (Affymetrix). All samples were prepared with the standard protocol recommended by the manufacturer.	Mice were maintained at all times on a 12-hour light-dark cycle in a specific pathogen-free environment with free access to rodent chow and water.	Trizol extraction of total RNA was performed according to the manufacturer's instructions	Arrays were scanned on an Affymetrix Scanner 3000-7G scanner with GCOS software. Scanning was peformed according to the protocol described in the Affymetrix GeneChip® Expression Analysis Technical Manual, November 2004 Edition.										
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol										
Experimental Factor Name	GENOTYPE															
Experimental Factor Type	genotype															
PubMed ID	24429522															
Comment[SecondaryAccession]	GSE43242															
Comment[GEOReleaseDate]	2014-01-21															
Comment[ArrayExpressSubmissionDate]	2013-01-02															
Comment[GEOLastUpdateDate]	2014-01-21															
Comment[AEExperimentType]	transcription profiling by array															
SDRF File	E-GEOD-43242.sdrf.txt