Comment[ArrayExpressAccession] E-GEOD-42582 MAGE-TAB Version 1.1 Public Release Date 2012-11-29 Investigation Title Oncorhynchus mykiss muscle cells: Small vs. Large fish Comment[Submitted Name] Oncorhynchus mykiss muscle cells: Small vs. Large fish Experiment Description Transcriptional profiling of rainbow trout muscle cells comparing muscle cells from small fish with muscle cells from large fish at two time periods. Two-condition experiment, small vs. large-fish muscle cells. Sept. and Dec. spawning fish. Biological replicates: 4 small replicates, 4 large replicates for each time period. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Kocmarek Kocmarek Ferguson Danzmann Person First Name Andrea Andrea Moira Roy Person Mid Initials Lynne L M G Person Email akocmare@uoguelph.ca Person Affiliation University of Guelph Person Phone 519-824-4120 Person Address Intergrative Biology, University of Guelph, Science Complex, University of Guelph, Guelph, Ontario, Canada Person Roles submitter Protocol Name P-GSE42582-1 P-GSE42582-6 P-GSE42582-5 P-GSE42582-3 P-GSE42582-2 P-GSE42582-4 P-GSE42582-7 Protocol Description ID_REF = VALUE = Lowess normalized log2 ratio of small fish compared to large fish Images were scanned on a GenePix 4200A scanner Images were quantified using Genepix Pro software (version 6.1). cDNA was hybridized according to Preventing Ozone Related Problems version of the the hybridization protocol described in Two-Color Microarray-Based Gene Expression Analysis (Agilent Technologies) in Agilent hybridization chambers. Total RNA extracted using Trizol following manufacturer's instructions mRNA was extracted from total RNA using M-NM-