Comment[ArrayExpressAccession]	E-GEOD-42552
MAGE-TAB Version	1.1								
Public Release Date	2013-07-03
Investigation Title	JA-INDUCIBLE MYC2-LIKE1 acts as a repressor and negatively regulates jasmonate signaling in Arabidopsis								
Comment[Submitted Name]	JA-INDUCIBLE MYC2-LIKE1 acts as a repressor and negatively regulates jasmonate signaling in Arabidopsis
Experiment Description	Jasmonate (JA) is a plant hormone that controls trade-offs between plant growth and responses to biotic and abiotic stresses. Although recent studies uncover core mechanism for JA-induced responses in Arabidopsis thaliana, it remains elusive how plants attenuate those responses. We report here that a basic-helix-loop-helix type transcription factor named JA-INDUCIBLE MYC2-LIKE1 (JAM1) acts as a transcriptional repressor and negatively regulates JA signaling. Arabidopsis plants expressing the chimeric repressor for JAM1 exhibited a substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by Spodoptera exigua. Conversely, jam1-4 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to the insect attack. Competitive binding of JAM1 and MYC2 to the target sequence of MYC2 suggested negative regulation of JA signaling by JAM1 and suppression of MYC2 function. These results indicate that JAM1 plays a pivotal role in fine-tuning of JA-mediated stress responses and plant growth by negatively regulating JA signaling. Transcriptomes of ProJAM1:JAM1-SRDX, ProMYC2:MYC2-SRDX and wild-type Arabidopsis seedlings with or without jasmonic acid were compared.								
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl							
Person Last Name	Mitsuda	Masaru	Nobutaka	Javier	Marco	Abraham	Gregg	Kaoru	Masaru
Person First Name	Nobuitaka	Nakata	Mitsuda	Moreno	Herde	Koo	Howe	Suzuki	Ohme
Person Mid Initials				E		J	A		T
Person Email	nobutaka.mitsuda@aist.go.jp								
Person Affiliation	National Institute of Advanced Industrial Science and Technology								
Person Address	Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Central 4 Higashi 1-1-1, Tsukuba, Ibaraki, Japan								
Person Roles	submitter								
Protocol Name	P-GSE42552-1	P-GSE42552-5	P-GSE42552-6	P-GSE42552-2	P-GSE42552-3	P-GSE42552-4	P-GSE42552-7		
Protocol Description	Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1). Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction. After that, the signal of each probe was divided by the median value of filter-passed probes. ID_REF =  VALUE = Normalized signal intensity (divided by median value)	200 ng of total RNA was used for the labeling procedure using the Low Input Quick Amp Labeling kit (One-color) (Agilent).	Hybridized using the Gene Expression Hybridization kit (Agilent) and washed by the Gene Expression wash pack (Agilent).	MeJA in DMSO or DMSO only was added to liquid medium in a final concentration of 50 microM and cultured 1 hour.	All plants were grown in MS (1% sucrose) liquid medium in a 16hr light / 8hr dark cycle in 23 Celsius degrees.	The Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.	Scanned on an Agilent G2505B scanner.		
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol		
Experimental Factor Name	TREATMENT	GENOTYPE							
Experimental Factor Type	treatment	genotype							
Publication Title	A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling in Arabidopsis.								
Publication Author List	Nakata M, Mitsuda N, Herde M, Koo AJ, Moreno JE, Suzuki K, Howe GA, Ohme-Takagi M								
PubMed ID	23673982								
Publication DOI	10.1105/tpc.113.111112								
Comment[SecondaryAccession]	GSE42552								
Comment[GEOReleaseDate]	2013-07-03								
Comment[ArrayExpressSubmissionDate]	2012-11-27								
Comment[GEOLastUpdateDate]	2013-07-03								
Comment[AEExperimentType]	transcription profiling by array								
SDRF File	E-GEOD-42552.sdrf.txt