Comment[ArrayExpressAccession]	E-GEOD-42507
MAGE-TAB Version	1.1						
Public Release Date	2013-05-24
Investigation Title	Cluster analysis reveals differential transcript profiles associated with resistance training-induced human skeletal muscle hypertrophy						
Comment[Submitted Name]	Cluster analysis reveals differential transcript profiles associated with resistance training-induced human skeletal muscle hypertrophy
Experiment Description	Overall, the widely disparate transcriptomes identified prior to RT among the three clusters support the notion that at least some of the inter-individual heterogeneity in propensity for RT-induced myofiber hypertrophy is likely pre-determined. Baseline comparisons of skeletal muscle transcript profiles from 44 subjects previously clustered (K-means cluster analysis) as non-, modest-, and extreme-responders (Non, n=15; Mod, n=14; Xtr, n=15) based on the magnitudes of change in vastus lateralis myofiber cross-sectional area (CSA) following 16-wk, 3-d/wk, high-intensity RT.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Thalacker-Mercer	Bamman	Thalacker-Mercer	Cui	Stec	Cross	Windham
Person First Name	Anna	Marcas	Anna	Xiangqin	Michael	James	Samuel
Person Email	aet74@cornell.edu						
Person Affiliation	Cornell University						
Person Phone	6072557007						
Person Fax	6072550130						
Person Address	Cornell University, 109 Savage Hall, Ithaca, USA						
Person Roles	submitter						
Protocol Name	P-GSE42507-1	P-GSE42507-4	P-GSE42507-5	P-GSE42507-2	P-GSE42507-3	P-GSE42507-6	
Protocol Description	Prior to normalization, genes with low signal intensity were removed. The data were then normalized using cubic spline and log transformed. ID_REF =  VALUE = normalized signal intensity	Two hundred nanograms of total RNA was converted into labeled cRNA with nucleotides coupled to fluorescent dye Cy3 using the Low Input Quick Amp Kit (Agilent Technologies, Palo Alto, CA) following the manufacturer's protocol.	Cy3-labeled cRNA (1.65 μg) from each sample was hybridized to an Agilent Human Whole Genome 4x44k Microarray	Baseline samples from a 16-wk, 3-d/wk, high-intensity resistance exercise training study.	Trizol followed by qiagen Rneasy clean-up	The hybridized array was then washed and scanned and data was extracted from the scanned image using Feature Extraction version 10.7 (Agilent Technologies).	
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol	
Experimental Factor Name	CLUSTER	SEX	AGE				
Experimental Factor Type	cluster	Sex	age				
Publication Title	Cluster analysis reveals differential transcript profiles associated with resistance training-induced human skeletal muscle hypertrophy.						
Publication Author List	Thalacker-Mercer A, Stec M, Cui X, Cross J, Windham ST, Bamman MM						
PubMed ID	23632419						
Publication DOI	10.1152/physiolgenomics.00167.2012						
Comment[SecondaryAccession]	GSE42507						
Comment[GEOReleaseDate]	2013-05-24						
Comment[ArrayExpressSubmissionDate]	2012-11-26						
Comment[GEOLastUpdateDate]	2013-05-24						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-42507.sdrf.txt