Investigation Title	Transcription profiling of human pituitary non-invasive and invasive tumors	
Comment[Submitted Name]	Hussaini-2R01NS035122-06A1	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[ArrayExpressReleaseDate]	2008-06-12	
Comment[SecondaryAccession]	GSE4237	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-GEOD-4237	
Comment[MAGETAB TimeStamp_Version]	2010-08-05 14:03:11 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Salomon	
Person First Name	Elizabeth	
Person Mid Initials	
Person Email	esalomon@tgen.org	
Person Phone	
Person Fax	
Person Address	NIH Neuroscience Microarray Consortium, ,NIH Neuroscience Microarray Consortium,445 N. Fifth Street, 5th floor,Phoenix,85004,USA	
Person Affiliation	NIH Neuroscience Microarray Consortium	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-12	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	Pituitary adenomas are common benign neoplasms giving rise to disorders of growth, reproductive function and cortisol production. Although recently determined to be monoclonal, very little is known about the mechanisms regulating the development of pituitary hyperplasia and neoplasia in humans. Surgical resection is the treatment of choice for most symptomatic pituitary adenomas. The goal of surgery is the complete removal of the tumor and the success of surgery is strongly affected by the presence of local invasion. However, complete tumor removal is unlikely when there is extensive local invasion. Identifying genes that control the invasiveness and recurrence of this class of tumors will provide therapeutic targets for this class of tumors. We will determine the expression pattern of genes in recurrent and invasive and  pituitary adenomas and compare those to non-invasive and non-recurrent  tumors. We hypothesize that the differential expression and activation of a number of genes affect pituitary adenoma recurrence  and invasiveness. Rationale:  Preliminary result showed a differential expression of novel PKC isozymes in non-invasive and invasive pituitary adenomas. PMA, an activator of both novel and classical PKC isozymes increased the expression of gelatinase A (MMP-2) mRNA in human pituitary adenoma cell line. This result raises a fundamental question as to the functional role of novel PKC isozymes and proteases in the invasive phenotype of pituitary adenomas. The primary component of this Specific Aim is to determine whether specific genes are differentially expressed in recurrent or invasive adenomas when compared with control non-invasive tumors.  Tissue specimens from non-invasive and invasive (dural invasion based microscopic examination) and recurrent vs non-recurrent tumors will be used for microarray analysis.  Frozen pituitaryspecimens will be collected and total RNA extracted with TriZol reagent. We will  provide  total RNA (10 ug) from non-invasive, invasive, reoccurred and non-occurrent pituitary adenomas.	
Protocol Name	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	hybridization	feature_extraction	
Protocol Description	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software	MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF	mo	
SDRF File	E-GEOD-4237.sdrf.txt	
Term Source Name	mo	The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version