Comment[ArrayExpressAccession]	E-GEOD-41783
MAGE-TAB Version	1.1					
Public Release Date	2013-03-31
Investigation Title	IGROV1 miRNA expression analysis after in vivo locoregional treatment with CpG-ODN					
Comment[Submitted Name]	IGROV1 miRNA expression analysis after in vivo locoregional treatment with CpG-ODN
Experiment Description	We reported that peri-tumoral CpG-ODN treatment, probably activating TLR9-expressing cells present in the tumor microenvironment, sensitized cancer cells to DNA-damaging chemotherapy (Cancer Res 2011 Oct 15;71(20):6382-90). Here, we investigated whether this treatment induces a modulation of miRNAs and their involvement in chemotherapy sensitivity. Twenty miRNAs were found differentially expressed in tumors from CpG-ODN-treated mice versus controls. Evaluation of the role of miR-424, miR-340 and miR-302b on cisplatin sensitivity revealed that ectopic expression of miR-302b (up-modulated in our array) in IGROV1 cells significantly improved cisplatin activity. The identification of miRNAs able to modify sensitivity to chemotherapy treatment will provide an experimental base for its future possible use as a target or tool of specific therapies. IGROV1 human ovarian carcinoma cells were adapted to growth intraperitoneally (i.p.) and maintained by serial i.p. passages of ascitic cells into healthy mice. Mice were injected i.p. with 2.5 x 10^6 ascitic cells in 0.2 ml of saline and treated starting 10-11 days later, when mice showed evident and established ascites, with CpG-ODN [phosphorothioated ODN1826 (5'-TCCATGACGTTCCTGACGTT-3')] delivered i.p. at a dose of 20 µg/mouse for 3 consecutive days, control mice received saline (4 mice/group). 24 hours after the last treatment, ascites-bearing mice were sacrificed. Tumor cells adherent to peritoneal wall were collected and immediately frozen in liquid nitrogen until RNA extraction.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	De Cecco	De Cecco	Balsari	Berardi		
Person First Name	Loris	L	A	M		
Person Email	loris.dececco@istitutotumori.mi.it					
Person Affiliation	IRCSS Istituto Nazionale Tumori 					
Person Address	IRCSS Istituto Nazionale Tumori , via Venezian 1, Milan, Italy					
Person Roles	submitter					
Protocol Name	P-GSE41783-1	P-GSE41783-5	P-GSE41783-4	P-GSE41783-2	P-GSE41783-3	P-GSE41783-6
Protocol Description	ID_REF =  VALUE = Quantile-normalized signal intensity Detection Pval = 	Standard Illumina scanning protocol.	Standard Illumina hybridization protocol.	RNA was extracted with the QIAGEN miRNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.	Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.	The data were processed and normalized using quantile normalization with BeadStudio software.
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction
Experimental Factor Name	TREATMENT					
Experimental Factor Type	treatment					
Comment[SecondaryAccession]	GSE41783					
Comment[GEOReleaseDate]	2013-03-31					
Comment[ArrayExpressSubmissionDate]	2012-10-23					
Comment[GEOLastUpdateDate]	2013-03-31					
Comment[AEExperimentType]	transcription profiling by array					
Comment[AdditionalFile:Data1]	GSE41783_non-normalized.txt					
SDRF File	E-GEOD-41783.sdrf.txt