Comment[ArrayExpressAccession]	E-GEOD-41312
MAGE-TAB Version	1.1				
Public Release Date	2013-10-01
Investigation Title	Modeling Pathogenesis of Primary Liver Cancer in Lineage Specific Cell Types				
Comment[Submitted Name]	Modeling Pathogenesis of Primary Liver Cancer in Lineage Specific Cell Types
Experiment Description	Classification of human liver cancer into biologically distinct subgroups suggests origin from different hepatic lineage cells. To clarify contribution of lineage stage in liver oncogenesis, we transduced H-Ras and SV40LT into hepatic progenitor cells (HPC), hepatoblasts (HB) and terminally differentiated adult hepatocytes (AH). Regardless of origin, all transformed cell types acquired common cancer stem cell characteristics in vitro and in vivo. However, expression analysis distinguished tumors of different cellular origin underscoring the contribution of lineage/stage-dependent genetic changes in malignant transformation. Notably, AH-derived tumors showed specific enrichment of c-Myc target genes. These data establish that any hepatic lineage cell can be a target for transformation and acquire common cancer stem cell traits via activation of diverse cell type specific pathways. Profiling of individual tumors derived by Ras/SV40 transformation of hepatic progenitor cell (oval cells), hepatoblasts (fetal) and adult hepatocytes				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Andersen	Andersen	Holczbauer	Thorgeirsson	
Person First Name	Jesper	Jesper	Agnes	Snorri	
Person Mid Initials	B	B		S	
Person Email	geo@ncbi.nlm.nih.gov				
Person Affiliation	NIH				
Person Address	NCI, NIH, 37 Convent Drive, Bethesda, MD, USA				
Person Roles	submitter				
Protocol Name	P-GSE41312-1	P-GSE41312-3	P-GSE41312-4	P-GSE41312-2	P-GSE41312-5
Protocol Description	Data was processed by means of Genome Studio v2011 from illumina, Inc. ID_REF =  VALUE = Quantile normalized with background noise subtraction Detection Pval = 	Biotinylated cRNA were prepared using Ambion Totalprep RNA Amplification kit for Illumina arrays	Standard Illumina hybridization protocol	Total RNA was extracted using Qiagen mini kit. Quality control and measurement were performed using NanoDrop and Agilent Bioanalyser.	Standard Illumina scanning protocol
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL TYPE				
Experimental Factor Type	cell type				
Comment[SecondaryAccession]	GSE41312				
Comment[GEOReleaseDate]	2013-10-01				
Comment[ArrayExpressSubmissionDate]	2012-10-03				
Comment[GEOLastUpdateDate]	2013-10-02				
Comment[AEExperimentType]	transcription profiling by array				
Comment[AdditionalFile:Data1]	GSE41312_non-normalized.txt				
SDRF File	E-GEOD-41312.sdrf.txt