Comment[ArrayExpressAccession]	E-GEOD-41226
MAGE-TAB Version	1.1						
Public Release Date	2013-07-18
Investigation Title	Expression data from G9a knockdown ovarian cancer cell						
Comment[Submitted Name]	Expression data from G9a knockdown ovarian cancer cell
Experiment Description	G9a depleted SKCO3 cells were used to identify genes regulated by G9a-dependent epigenetic regulations We used microarrays to detail the global programme of gene expression underlying paritoneal dissemination and identified distinct classes of G9a-regulated genes during this process. SKOV3 cells receiving G9a-specific shRNAs were analyzed compare to cells receiving control shRNA						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	hua	Hua	Kuo	Yen			
Person First Name	kuo-tai	Kuo-Tai	Min-Liang	Men-Luh			
Person Email	d94447003@gmail.com						
Person Affiliation	National Taiwan University						
Person Address	National Taiwan University, No.2, Xu-Zhou Road, Taipei , Taiwan						
Person Roles	submitter						
Protocol Name	P-GSE41226-1	P-GSE41226-5	P-GSE41226-6	P-GSE41226-2	P-GSE41226-3	P-GSE41226-4	P-GSE41226-7
Protocol Description	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. ID_REF =  VALUE = MAS5 Signal ABS_CALL = indicating whether the transcript was present (P), absent (A), or marginal (M) DETECTION P-VALUE = 	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.	Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.	Cells were cultured in 5% CO2 incubator at 37℃.	Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	GENOTYPE						
Experimental Factor Type	genotype						
Comment[SecondaryAccession]	GSE41226						
Comment[GEOReleaseDate]	2013-07-18						
Comment[ArrayExpressSubmissionDate]	2012-09-28						
Comment[GEOLastUpdateDate]	2013-07-24						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-41226.sdrf.txt