Comment[ArrayExpressAccession] E-GEOD-39385
Public Release Date 2012-07-16
Investigation Title The AtGenExpress: hormone inhibitor or other chemical treatment of seedlings in Arabidopsis
Comment[Submitted Name] The AtGenExpress: hormone inhibitor or other chemical treatment of seedlings in Arabidopsis
Experiment Description Arabidopsis seedlings of the wild-type were treated with inhibitors. 60 samples were analyzed by Affymetrix GeneChip, ATH1. Sample correspondence between control (mock) and inhibitor treatment is described in brackets (F series or G series).
Date of Experiment
Term Source Name EFO
Term Source Version
Term Source File http://www.ebi.ac.uk/efo/efo.owl
Person Last Name Shimada Shimada
Person First Name Yukihisa Yukihisa
Person Mid Initials
Person Email yshimada@yokohama-cu.ac.jp
Person Affiliation Yokohama City University
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Person Address Kihara Institute for Biological Science, Yokohama City University, Maiokacho, Totsuka, Yokohama, Kanagawa, Japan
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Protocol Name P-GSE39385-1 P-GSE39385-6 P-GSE39385-3 P-GSE39385-8 P-GSE39385-7 P-GSE39385-2 P-GSE39385-4 P-GSE39385-5
Protocol Description ID_REF =
VALUE = MAS5
ABS_CALL =
Detection p-value = Affymetrix standard protocol Seedlings were grown for 7 days at 23 degrees. MAS5.0 Affymetrix Gene Array Scanner 2500A with the original setting (This means that scanning steps are conducted at a high level of sensitivity to focus on those genes that function in hormone signaling, because their transcripts are relatively less abundant. Under these conditions, signals of highly abundant transcripts may reach saturation) Seedlings were treated with an inhibitor for 3 h or 12h. guanidine-hydrochloride method One-cycle target labeling reagents (Affymetrix)
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Protocol Type bioassay_data_transformation hybridization grow feature_extraction image_aquisition specified_biomaterial_action nucleic_acid_extraction labeling
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Experimental Factor Name TREATMENT TIME
Experimental Factor Type treatment time
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Comment[SecondaryAccession] GSE39385
Comment[GEOLastUpdateDate] 2012-07-17
Comment[AEExperimentType] transcription profiling by array
Comment[GEOReleaseDate] 2012-07-15
Comment[ArrayExpressSubmissionDate] 2012-07-15
SDRF File E-GEOD-39385.sdrf.txt