Comment[ArrayExpressAccession] E-GEOD-39385 Public Release Date 2012-07-16 Investigation Title The AtGenExpress: hormone inhibitor or other chemical treatment of seedlings in Arabidopsis Comment[Submitted Name] The AtGenExpress: hormone inhibitor or other chemical treatment of seedlings in Arabidopsis Experiment Description Arabidopsis seedlings of the wild-type were treated with inhibitors. 60 samples were analyzed by Affymetrix GeneChip, ATH1. Sample correspondence between control (mock) and inhibitor treatment is described in brackets (F series or G series). Date of Experiment Term Source Name EFO Term Source Version Term Source File http://www.ebi.ac.uk/efo/efo.owl Person Last Name Shimada Shimada Person First Name Yukihisa Yukihisa Person Mid Initials Person Email yshimada@yokohama-cu.ac.jp Person Affiliation Yokohama City University Person Phone Person Fax Person Address Kihara Institute for Biological Science, Yokohama City University, Maiokacho, Totsuka, Yokohama, Kanagawa, Japan Person Roles submitter Person Roles Term Source REF Person Roles Term Accession Number Normalization Type Normalization Term Accession Number Normalization Term Source REF Replicate Type Replicate Term Accession Number Replicate Term Source REF Experimental Design Experimental Design Term Accession Number Experimental Design Term Source REF Quality Control Type Quality Control Term Accession Number Quality Control Term Source REF Protocol Name P-GSE39385-1 P-GSE39385-6 P-GSE39385-3 P-GSE39385-8 P-GSE39385-7 P-GSE39385-2 P-GSE39385-4 P-GSE39385-5 Protocol Description ID_REF =
VALUE = MAS5
ABS_CALL =
Detection p-value = Affymetrix standard protocol Seedlings were grown for 7 days at 23 degrees. MAS5.0 Affymetrix Gene Array Scanner 2500A with the original setting (This means that scanning steps are conducted at a high level of sensitivity to focus on those genes that function in hormone signaling, because their transcripts are relatively less abundant. Under these conditions, signals of highly abundant transcripts may reach saturation) Seedlings were treated with an inhibitor for 3 h or 12h. guanidine-hydrochloride method One-cycle target labeling reagents (Affymetrix) Protocol Software Protocol Hardware Protocol Contact Protocol Type bioassay_data_transformation hybridization grow feature_extraction image_aquisition specified_biomaterial_action nucleic_acid_extraction labeling Protocol Term Source REF Protocol Term Accession Number Experimental Factor Name TREATMENT TIME Experimental Factor Type treatment time Experimental Factor Term Source REF Experimental Factor Term Accession Number Publication Title Publication Author List PubMed ID Publication DOI Publication Status Publication Status Term Source REF Publication Status Term Accession Number Comment[SecondaryAccession] GSE39385 Comment[GEOLastUpdateDate] 2012-07-17 Comment[AEExperimentType] transcription profiling by array Comment[GEOReleaseDate] 2012-07-15 Comment[ArrayExpressSubmissionDate] 2012-07-15 SDRF File E-GEOD-39385.sdrf.txt