Comment[ArrayExpressAccession]	E-GEOD-34835
MAGE-TAB Version	1.1				
Public Release Date	2013-06-03
Investigation Title	Role and function of Bcor in BCR-ABL1 driven pre-B cells				
Comment[Submitted Name]	Role and function of Bcor in BCR-ABL1 driven pre-B cells
Experiment Description	In order to investigate the function of Bcor in ALL, we isolated bone marrow cells from Bcor fl/Y mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with CRE-ERT2 or ERT2 (PMIP) and after 2 days of treatment with 4OH-Tamoxifen (0.5 micromolar) subjected to gene expression analysis.  Two days after treatment with 4OH-Tamoxifen (0.5 micromolar) total RNA of Puromycin resistant cells was extracted and subjected to gene expression analysis. 				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Müschen	shojaee			
Person First Name	Markus	seyedmehdi			
Person Email	mmuschen@chla.usc.edu				
Person Affiliation	University of Southern California				
Person Phone	+ 1 323 361 5592				
Person Fax	+1 323 361 1516				
Person Address	Childrens Hospital Los Angeles, University of Southern California, 4650 Sunset Blvd, Los Angeles, CA, USA				
Person Roles	submitter				
Protocol Name	P-GSE34835-1	P-GSE34835-3	P-GSE34835-4	P-GSE34835-2	P-GSE34835-5
Protocol Description	RMA- Partek Genomics suite 6.5 ID_REF =  VALUE = RMA-calculated signal intensities	Standard Affymetrix protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site.	Standard Affymetrix protocols (see GeneChip Expression Analysis Technical Manual)	Qiagen_RNeasy_Kit	Standard Affymetrix protocols (see GeneChip Expression Analysis Technical Manual)
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	TRANSDUCTION				
Experimental Factor Type	transduction				
Comment[SecondaryAccession]	GSE34835				
Comment[GEOReleaseDate]	2013-06-03				
Comment[ArrayExpressSubmissionDate]	2012-01-03				
Comment[GEOLastUpdateDate]	2013-06-03				
Comment[AEExperimentType]	transcription profiling by array				
SDRF File	E-GEOD-34835.sdrf.txt