Comment[ArrayExpressAccession] E-GEOD-34080 MAGE-TAB Version 1.1 Public Release Date 2013-12-02 Investigation Title Expression profiling on HPSE deleted SGC-7901 gastric cancer cells Comment[Submitted Name] Expression profiling on HPSE deleted SGC-7901 gastric cancer cells Experiment Description HPSE plays important roles in gastric cancer cell proliferation, apoptosis and metastasis.The aim of this study is to explore molecular mechanism underling roles of HPSE in gastric cancer cell proliferation, survival, migration and metastasis. SGC-7901 gastric cancer cells were transfected with HPSE siRNA (10nM) or scramble control siRNA, RNA were extracted 24hours after transfectioin and hybridized to Affymetrix microarrays. 3 biological repeats were used for each condition. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Zhang Cai Chen Lu luo Zhang Zhao Zhang Chen Person First Name Yan Aizhen Shaoquan Canrong Yuan Lezhi Zhihu Yan Lin Person Email zany1983@gmail.com Person Affiliation Beijing institute of Biotechnology Person Phone 861066948775 Person Address Beijing institute of Biotechnology, Dongdajie street, Beijing, Not Applicable, China Person Roles submitter Protocol Name P-GSE34080-1 P-GSE34080-5 P-GSE34080-6 P-GSE34080-2 P-GSE34080-3 P-GSE34080-4 P-GSE34080-7 Protocol Description The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. A log base 2 transformation is applied to the data before the arrays are normalized. The trimmed mean target intensity of each array was arbitrarily set to 100. ID_REF = VALUE = MAS5.0 signal intensity Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix). Standard Affymetrix protocol SGC-7901 cells were transiently transfected with HPSE siRNA or scramble control siRNA using lipofectamine 2000(life technology), according to the manufacturerM-bM-^@M-^Ys instructions.. 24hours later, cells were collected in Trizol solution (life technology). cells were grown in RPMI1640 medium (Life Technologies, Inc., Gaithersburg,MD) supplemented with 10% fetal bovine serum (FBS, Hyclone, Inc.), penicillin (100 U/ml) and streptomycin (100 M-NM-