Comment[ArrayExpressAccession]	E-GEOD-33455							
Public Release Date	2012-01-01							
Investigation Title	Expression data from docetaxel-resistant prostate cancer cell lines							
Comment[Submitted Name]	Expression data from docetaxel-resistant prostate cancer cell lines							
Experiment Description	Docetaxel-based chemotherapy is the standard first-line therapy in metastatic castration-resistant prostate cancer. However, most patients eventually develop resistance to this treatment. The aim of the study was to identify key molecular genes and networks associated with docetaxel resistance in 2 models of docetaxel-resistant castration-resistant prostate cancer cell lines. DU-145 and PC-3 cells were converted to docetaxel-resistant cells, DU-145R and PC-3R, respectively. Whole-genome arrays were used to compare global gene expression between these 4 cell lines. Arrays were performed by triplicate for each cell line.							
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl							
Person Last Name	Marin-Aguilera	Marin-Aguilera	Kalko	Mellado				
Person First Name	Mercedes	Mercedes	Susana	Begona				
Person Mid Initials			G					
Person Email	mmarin1@clinic.ub.es							
Person Affiliation	Fundacio Clinic per a la Recerca Biomedica							
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Person Address	Fundacio Clinic per a la Recerca Biomedica, Villarroel 170, Barcelona, Spain							
Person Roles	submitter							
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Protocol Name	P-GSE33455-1	P-GSE33455-6	P-GSE33455-3	P-GSE33455-8	P-GSE33455-7	P-GSE33455-2	P-GSE33455-4	P-GSE33455-5
Protocol Description	ID_REF = <br>VALUE = RMA summarized log2 values	GeneChip Hybridization wash and stain kit	DU-145 and DU-145R cell lines were cultured in RPMI 1640 medium (Invitrogen), and PC-3 and PC-3R in F-12K nutrient mixture medium (Invitrogen), both supplemented with 10% fetal bovine serum.	Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the affy package from the Bioconductor project.	GeneChip 3000 Scanner 3000 7G system; software: GeneChip Command Console (AGCC 1.1)	The human prostate carcinoma cell lines DU-145 and PC-3 (obtained from American Tissue Culture Collection) were converted to docetaxel-resistant cells by exposing them to an initial dose of 5 nM docetaxel and culturing surviving cells during 1 yr and 6 mo, respectively, with increasing doses in an intermittent regimen. Docetaxel (Sigma) was dissolved in DMSO (10mM).	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	Biotinylated cRNA were prepared according to the Affymetrix IVT Labeling kit from 2ug total RNA.
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Protocol Type	bioassay_data_transformation	hybridization	grow	feature_extraction	image_aquisition	specified_biomaterial_action	nucleic_acid_extraction	labeling
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Experimental Factor Name	cell line	treatment						
Experimental Factor Type	cell line	treatment						
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Publication Title	Identification of docetaxel resistance genes in castration-resistant prostate cancer.							
Publication Author List	Marin-Aguilera M, Codony-Servat J, Kalko SG, Fernandez PL, Bermudo R, Buxo E, Ribal MJ, Gascon P, Mellado B							
PubMed ID	22027694							
Publication DOI	10.1158/1535-7163.MCT-11-0289							
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Comment[SecondaryAccession]	GSE33455							
Comment[GEOLastUpdateDate]	2012-06-14							
Comment[AEExperimentType]	transcription profiling by array							
Comment[GEOReleaseDate]	2012-01-01							
Comment[ArrayExpressSubmissionDate]	2011-11-03							
SDRF File	E-GEOD-33455.sdrf.txt