Investigation Title	Transcription profiling of seven consecutive stages of mouse thymocyte development	
Comment[Submitted Name]	T and B lymphocyte development profiles	
Experimental Design	development_or_differentiation_design	cell_type_comparison_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo:1.3.1.1	mo	mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-12-20	
Comment[SecondaryAccession]	GSE272	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-GEOD-272	
Comment[MAGETAB TimeStamp_Version]	2011-01-22 16:34:55 Last Changed Rev: 14857	
Experimental Factor Name	Phenotype	CellType	
Experimental Factor Type	phenotype	cell_type	
Experimental Factor Term Source REF	
Person Last Name	Hoffmann	
Person First Name	Reinhard	
Person Mid Initials	
Person Email	r_hoffmann@mvp.uni-muenchen.de	
Person Phone	
Person Fax	
Person Address	Bacteriology, Max von Pettenkofer Institut, Pettenkoferstr. 9a, Munich, 80336, Germany	
Person Affiliation	Max von Pettenkofer Institut	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-12-20	
PubMed ID	12538694	
Publication DOI	12538694	
Publication Author List	Reinhard Hoffmann, Ludovica Bruno, Thomas Seidl, Antonius Rolink, Fritz Melchers	
Publication Title	Rules for gene usage inferred from a comparison of large-scale gene expression profiles of T and B lymphocyte development.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	Expression profiles of seven consecutive stages of mouse thymocyte development were generated.	
Protocol Name	P-GEOD-272-115	P-GEOD-272-19	P-GEOD-272-100	P-GEOD-272-38	P-GEOD-272-6	P-GEOD-272-41	P-GEOD-272-54	P-GEOD-272-123	P-GEOD-272-65	P-GEOD-272-118	P-GEOD-272-129	P-GEOD-272-13	P-GEOD-272-96	P-GEOD-272-80	P-GEOD-272-15	P-GEOD-272-56	P-GEOD-272-28	P-GEOD-272-18	P-GEOD-272-107	P-GEOD-272-76	P-GEOD-272-94	P-GEOD-272-7	P-GEOD-272-25	P-GEOD-272-101	P-GEOD-272-99	P-GEOD-272-39	P-GEOD-272-24	P-GEOD-272-69	P-GEOD-272-60	P-GEOD-272-79	P-GEOD-272-77	P-GEOD-272-135	P-GEOD-272-138	P-GEOD-272-122	P-GEOD-272-64	P-GEOD-272-111	P-GEOD-272-40	P-GEOD-272-125	P-GEOD-272-102	P-GEOD-272-4	P-GEOD-272-20	P-GEOD-272-21	P-GEOD-272-105	P-GEOD-272-31	P-GEOD-272-33	P-GEOD-272-75	P-GEOD-272-43	P-GEOD-272-49	P-GEOD-272-78	P-GEOD-272-17	P-GEOD-272-1	P-GEOD-272-48	P-GEOD-272-112	P-GEOD-272-61	P-GEOD-272-93	P-GEOD-272-2	P-GEOD-272-110	P-GEOD-272-53	P-GEOD-272-89	P-GEOD-272-14	P-GEOD-272-62	P-GEOD-272-132	P-GEOD-272-108	P-GEOD-272-134	P-GEOD-272-22	P-GEOD-272-127	P-GEOD-272-34	P-GEOD-272-130	P-GEOD-272-121	P-GEOD-272-3	P-GEOD-272-16	P-GEOD-272-126	P-GEOD-272-120	P-GEOD-272-27	P-GEOD-272-50	P-GEOD-272-124	P-GEOD-272-12	P-GEOD-272-67	P-GEOD-272-51	P-GEOD-272-58	P-GEOD-272-52	P-GEOD-272-109	P-GEOD-272-137	P-GEOD-272-30	P-GEOD-272-103	P-GEOD-272-42	P-GEOD-272-106	P-GEOD-272-113	P-GEOD-272-91	P-GEOD-272-86	P-GEOD-272-9	P-GEOD-272-84	P-GEOD-272-46	P-GEOD-272-68	P-GEOD-272-63	P-GEOD-272-97	P-GEOD-272-72	P-GEOD-272-81	P-GEOD-272-57	P-GEOD-272-87	P-GEOD-272-26	P-GEOD-272-11	P-GEOD-272-95	P-GEOD-272-83	P-GEOD-272-70	P-GEOD-272-131	P-GEOD-272-98	P-GEOD-272-32	P-GEOD-272-59	P-GEOD-272-114	P-GEOD-272-136	P-GEOD-272-82	P-GEOD-272-36	P-GEOD-272-47	P-GEOD-272-35	P-GEOD-272-37	P-GEOD-272-73	P-GEOD-272-44	P-GEOD-272-128	P-GEOD-272-66	P-GEOD-272-133	P-GEOD-272-23	P-GEOD-272-8	P-GEOD-272-119	P-GEOD-272-55	P-GEOD-272-71	P-GEOD-272-90	P-GEOD-272-88	P-GEOD-272-5	P-GEOD-272-117	P-GEOD-272-74	P-GEOD-272-92	P-GEOD-272-10	P-GEOD-272-85	P-GEOD-272-116	P-GEOD-272-104	P-GEOD-272-45	P-GEOD-272-29	
Protocol Type	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	
Protocol Description	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 2	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 5852.99, Stdev: 601.98, Max: 6678.5, Min: 4958.2 Corner+ Avg:3704, Count:32 Corner- Avg:41194, Count:32 BF= SDT=348.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=87.13 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=348.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 1	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 9	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. MAT3-2	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 9	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3344.96, Stdev: 178.34, Max: 3685.8, Min: 3090.2 Corner+ Avg:4299, Count:32 Corner- Avg:43068, Count:32 BF= SDT=244.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=61.06 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=244.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 1	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4173.69, Stdev: 418.28, Max: 4689.2, Min: 3231.2 Corner+ Avg:2955, Count:32 Corner- Avg:41638, Count:32 BF= SDT=304.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=76.00 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=304.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4093.98, Stdev: 497.30, Max: 4942.8, Min: 3321.0 Corner+ Avg:1242, Count:32 Corner- Avg:41039, Count:32 BF= SDT=289.2(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=72.29 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=289.2 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 3	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 10   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4247.77, Stdev: 357.06, Max: 4853.4, Min: 3714.6 Corner+ Avg:6086, Count:32 Corner- Avg:41351, Count:32 BF= SDT=297.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=74.47 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=297.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 1	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 10	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3136.57, Stdev: 248.40, Max: 3406.1, Min: 2570.9 Corner+ Avg:3725, Count:32 Corner- Avg:42947, Count:32 BF= SDT=289.2(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=72.29 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=289.2 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1373.86, Stdev: 57.48, Max: 1507.8, Min: 1286.1 Corner+ Avg:1397, Count:32 Corner- Avg:44773, Count:32 BF= SDT=157.8(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=39.45 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=157.8 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 6   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4557.15, Stdev: 289.20, Max: 5246.4, Min: 4164.5 Corner+ Avg:4991, Count:32 Corner- Avg:41172, Count:32 BF= SDT=298.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=74.61 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=298.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 1	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 3	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1546.40, Stdev: 88.99, Max: 1669.9, Min: 1346.1 Corner+ Avg:5564, Count:32 Corner- Avg:44572, Count:32 BF= SDT=163.2(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=40.80 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=163.2 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1828.19, Stdev: 76.29, Max: 1954.4, Min: 1702.4 Corner+ Avg:6334, Count:32 Corner- Avg:44381, Count:32 BF= SDT=177.6(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=44.41 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=177.6 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 11	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 10	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3623.69, Stdev: 333.61, Max: 4067.8, Min: 3058.5 Corner+ Avg:1526, Count:32 Corner- Avg:37096, Count:32 BF= SDT=270.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=67.51 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=270.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1154.59, Stdev: 46.72, Max: 1226.3, Min: 1066.6 Corner+ Avg:4059, Count:32 Corner- Avg:45041, Count:32 BF= SDT=133.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=33.48 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=133.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3496.93, Stdev: 913.98, Max: 5136.6, Min: 1936.0 Corner+ Avg:27150, Count:32 Corner- Avg:41874, Count:32 BF= SDT=266.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=66.59 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=266.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1139.56, Stdev: 40.81, Max: 1208.2, Min: 1059.4 Corner+ Avg:1652, Count:32 Corner- Avg:45057, Count:32 BF= SDT=141.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=35.25 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=141.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1890.85, Stdev: 83.65, Max: 2008.9, Min: 1739.3 Corner+ Avg:6500, Count:32 Corner- Avg:44310, Count:32 BF= SDT=171.1(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=42.78 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=171.1 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 3	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4233.10, Stdev: 364.30, Max: 4798.9, Min: 3742.6 Corner+ Avg:4046, Count:32 Corner- Avg:42358, Count:32 BF= SDT=281.2(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=70.29 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=281.2 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 2	B220+c-kit+ Pre BIcells sorted out of mouse bone marrow, sort no. 3	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3473.73, Stdev: 140.93, Max: 3654.9, Min: 3163.7 Corner+ Avg:4073, Count:32 Corner- Avg:42664, Count:32 BF= SDT=284.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=71.07 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=284.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 8	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 1	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1597.19, Stdev: 372.30, Max: 1953.1, Min: 1052.5 Corner+ Avg:795, Count:32 Corner- Avg:15554, Count:32 BF= SDT=175.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=43.76 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=175.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 9	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 9	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2653.25, Stdev: 676.61, Max: 3888.6, Min: 1456.2 Corner+ Avg:8961, Count:32 Corner- Avg:43057, Count:32 BF= SDT=213.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=53.37 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=213.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 9	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 1	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1305.47, Stdev: 46.85, Max: 1397.4, Min: 1219.9 Corner+ Avg:3418, Count:32 Corner- Avg:44896, Count:32 BF= SDT=151.7(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=37.93 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=151.7 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 6   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2041.23, Stdev: 51.09, Max: 2118.0, Min: 1907.1 Corner+ Avg:4455, Count:32 Corner- Avg:44152, Count:32 BF= SDT=189.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=47.24 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=189.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Immature B cells cells sorted out of mouse bone marrow, sort no. 1	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2916.24, Stdev: 154.48, Max: 3168.2, Min: 2531.2 Corner+ Avg:3374, Count:32 Corner- Avg:43100, Count:32 BF= SDT=268.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=67.12 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=268.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 6   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1187.76, Stdev: 54.22, Max: 1329.2, Min: 1100.8 Corner+ Avg:3903, Count:32 Corner- Avg:45034, Count:32 BF= SDT=142.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=35.61 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=142.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 1_2	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1329.04, Stdev: 33.92, Max: 1382.6, Min: 1266.1 Corner+ Avg:1237, Count:32 Corner- Avg:43513, Count:32 BF= SDT=156.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=39.07 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=156.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2795.25, Stdev: 175.00, Max: 3188.2, Min: 2583.8 Corner+ Avg:3285, Count:32 Corner- Avg:43240, Count:32 BF= SDT=257.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=64.34 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=257.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 9	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 10	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 11	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 9	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 1	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 9	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2539.54, Stdev: 88.34, Max: 2669.1, Min: 2383.2 Corner+ Avg:1711, Count:32 Corner- Avg:43503, Count:32 BF= SDT=197.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=49.47 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=197.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 10	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 10   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4054.31, Stdev: 189.65, Max: 4536.5, Min: 3774.8 Corner+ Avg:7031, Count:32 Corner- Avg:42226, Count:32 BF= SDT=288.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=72.23 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=288.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 3	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3965.52, Stdev: 301.43, Max: 4473.0, Min: 3604.3 Corner+ Avg:3836, Count:32 Corner- Avg:41824, Count:32 BF= SDT=320.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=80.00 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=320.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 2	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1441.30, Stdev: 69.90, Max: 1562.4, Min: 1301.8 Corner+ Avg:5389, Count:32 Corner- Avg:44799, Count:32 BF= SDT=156.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=39.08 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=156.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3327.17, Stdev: 164.46, Max: 3623.1, Min: 2973.7 Corner+ Avg:4878, Count:32 Corner- Avg:42641, Count:32 BF= SDT=255.1(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=63.78 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=255.1 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4737.75, Stdev: 373.89, Max: 5366.5, Min: 4091.9 Corner+ Avg:7609, Count:32 Corner- Avg:42001, Count:32 BF= SDT=312.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=77.99 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=312.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 6   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3790.67, Stdev: 348.84, Max: 4330.5, Min: 3243.6 Corner+ Avg:3943, Count:32 Corner- Avg:41955, Count:32 BF= SDT=285.8(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=71.46 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=285.8 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3439.30, Stdev: 439.18, Max: 4328.3, Min: 2802.2 Corner+ Avg:1313, Count:32 Corner- Avg:41950, Count:32 BF= SDT=285.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=71.38 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=285.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 1_2	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2706.96, Stdev: 82.72, Max: 2893.0, Min: 2596.6 Corner+ Avg:802, Count:32 Corner- Avg:43268, Count:32 BF= SDT=249.7(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=62.42 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=249.7 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 2	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1211.35, Stdev: 56.97, Max: 1308.0, Min: 1143.7 Corner+ Avg:1113, Count:32 Corner- Avg:43227, Count:32 BF= SDT=145.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=36.34 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=145.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 5127.65, Stdev: 246.59, Max: 5535.3, Min: 4735.0 Corner+ Avg:2896, Count:32 Corner- Avg:41062, Count:32 BF= SDT=307.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=76.97 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=307.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1975.24, Stdev: 63.60, Max: 2102.0, Min: 1868.8 Corner+ Avg:6662, Count:32 Corner- Avg:44230, Count:32 BF= SDT=178.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=44.60 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=178.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1616.36, Stdev: 42.93, Max: 1688.8, Min: 1549.3 Corner+ Avg:1814, Count:32 Corner- Avg:43172, Count:32 BF= SDT=171.8(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=42.95 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=171.8 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1636.09, Stdev: 62.29, Max: 1743.6, Min: 1535.9 Corner+ Avg:4049, Count:32 Corner- Avg:44572, Count:32 BF= SDT=166.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=41.59 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=166.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 10   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4781.68, Stdev: 174.60, Max: 5076.3, Min: 4488.5 Corner+ Avg:7902, Count:32 Corner- Avg:41320, Count:32 BF= SDT=285.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=71.49 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=285.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1044.14, Stdev: 44.52, Max: 1113.8, Min: 971.1 Corner+ Avg:1496, Count:32 Corner- Avg:45202, Count:32 BF= SDT=126.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=31.50 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=126.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 8	B220+CD25+sIg- Large Pre BII sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25+CD4-CD8- Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1491.69, Stdev: 61.45, Max: 1570.1, Min: 1367.1 Corner+ Avg:5648, Count:32 Corner- Avg:44699, Count:32 BF= SDT=155.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=38.86 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=155.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1355.16, Stdev: 65.52, Max: 1446.6, Min: 1213.8 Corner+ Avg:5686, Count:32 Corner- Avg:44853, Count:32 BF= SDT=148.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=37.10 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=148.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1167.88, Stdev: 69.04, Max: 1272.1, Min: 1041.1 Corner+ Avg:1178, Count:32 Corner- Avg:41438, Count:32 BF= SDT=138.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=34.62 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=138.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4279.53, Stdev: 140.19, Max: 4583.1, Min: 3988.8 Corner+ Avg:2863, Count:32 Corner- Avg:42000, Count:32 BF= SDT=276.8(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=69.19 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=276.8 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 9	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 10   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3439.00, Stdev: 141.29, Max: 3727.0, Min: 3238.6 Corner+ Avg:9415, Count:32 Corner- Avg:42843, Count:32 BF= SDT=258.2(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=64.55 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=258.2 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 9   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3148.08, Stdev: 222.97, Max: 3623.5, Min: 2839.1 Corner+ Avg:3219, Count:32 Corner- Avg:42627, Count:32 BF= SDT=275.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=68.87 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=275.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4374.44, Stdev: 149.54, Max: 4602.1, Min: 4164.7 Corner+ Avg:3030, Count:32 Corner- Avg:41689, Count:32 BF= SDT=300.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=75.13 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=300.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 8	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 3	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 2	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 9	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 4883.75, Stdev: 441.52, Max: 5451.9, Min: 3935.0 Corner+ Avg:3775, Count:32 Corner- Avg:42261, Count:32 BF= SDT=320.9(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=80.24 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=320.9 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+c-kit+ Pre BI cells sorted out of mouse bone marrow, sort no. 1	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 981.68, Stdev: 46.28, Max: 1079.0, Min: 898.8 Corner+ Avg:1688, Count:32 Corner- Avg:44969, Count:32 BF= SDT=117.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=29.34 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=117.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 3   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220+CD25+sIg- Small Pre BII cells sorted out of mouse bone marrow, sort no. 1	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 10	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	B220hi sIgM+ Mature B cells sorted out of mouse bone marrow, sort no. 11	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 2351.66, Stdev: 83.89, Max: 2462.8, Min: 2172.8 Corner+ Avg:967, Count:32 Corner- Avg:39645, Count:32 BF= SDT=221.7(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=55.42 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=221.7 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+large Replicate number: 6   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3079.34, Stdev: 160.35, Max: 3433.5, Min: 2916.8 Corner+ Avg:3762, Count:32 Corner- Avg:42761, Count:32 BF= SDT=240.8(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=60.19 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=240.8 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4-CD8+ Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1788.02, Stdev: 66.03, Max: 1902.7, Min: 1675.9 Corner+ Avg:1427, Count:32 Corner- Avg:44468, Count:32 BF= SDT=179.2(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=44.80 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=179.2 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 8	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44+CD25-CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 4   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1214.04, Stdev: 36.87, Max: 1272.8, Min: 1147.3 Corner+ Avg:1781, Count:32 Corner- Avg:44933, Count:32 BF= SDT=130.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=32.57 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=130.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4+CD8+small Replicate number: 6   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 3463.79, Stdev: 228.91, Max: 3915.7, Min: 3146.0 Corner+ Avg:7151, Count:32 Corner- Avg:42547, Count:32 BF= SDT=247.4(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=61.84 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=247.4 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+CD25+sIg- Large Pre BII cells sorted out of mouse bone marrow, sort no. 2	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD25-CD44-CD4+CD8- Replicate number: 5   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 1440.48, Stdev: 121.77, Max: 1770.0, Min: 1328.8 Corner+ Avg:2212, Count:32 Corner- Avg:44502, Count:32 BF= SDT=158.0(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=39.51 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=158.0 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25+CD4-CD8- Replicate number: 2   (Note: all samples with identical replicate numbers are derived from the same vial of cells).	Flow cytometrically purified T cell precursors of C57BL/6 mice. RNA was labeled by double round in vitro transcription-based RNA amplification. Images were analyzed with Affymetrix MAS 4.0. Files contain unnormalized AvgDiff values. Surface marker phenotype: CD44-CD25-CD4-CD8- Replicate number: 7   (Note: all samples with identical replicate numbers are derived from the same vial of cells). Background Avg: 5318.00, Stdev: 205.71, Max: 5626.0, Min: 4887.7 Corner+ Avg:2859, Count:32 Corner- Avg:40598, Count:32 BF= SDT=329.8(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=82.45 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=329.8 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 NF=1.000000 SF=1.000000	B220+c-kit+ Pre BIcells sorted out of mouse bone marrow, sort no. 10	B220Low sIgM+ immature B cells sorted out of mouse bone marrow, sort no. 11	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-272.sdrf.txt	
Term Source Name	efo.obo		The MGED Ontology	ArrayExpress	mo:1.3.1.1	mo	EFO	The MGED Ontology	
Term Source File	/nfs/ma/ma-exp/GEOImportDownload/efo.obo		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version					1.3.1.1