Comment[ArrayExpressAccession]	E-GEOD-2342
Public Release Date	2006-02-25					
Investigation Title	Dexamethasone responses in BRC-Abl (p210) transformed murine B-cells					
Comment[Submitted Name]	Dexamethasone responses in BRC-Abl (p210) transformed murine B-cells
Experiment Description	For generation of pre-B cell lines, bone marrow from tibias and femurs of 4 to 8 week old C56Bl/6 mice (wt and vav-Gfi1b transgene) was infected with retroviral stocks of different BCR-Abl constructs (MSCV-GFP-IRESp210 or pSRa-p210-tkneo) and plated at a density of 5E+06 per 6 cm dish. All pre-B cells were fed twice a week and analyzed after they became stroma independent. Cells received 1E-08 M dexamethasone or vehicle for 12 hours prior to RNA extraction using TRIzol. mRNAs were converted into doublestranded cDNA using a T7-promoter tagged oligodT primer and transcribed by T7 RNA polymerase in the presence of biotinylated NTPs. Targets were hybridized to Affymetrix MOE430A arrays, washed, stained and scanned (GeneChip scanner 2500) as recommended by the manufacturer. Images were processed either by MAS5.0 (global scaling, TGT=1000) or RMAExpress (quantile normalization and background substraction) software tools. By comparing the gene expression profiles of dexamethasone and non-induced cells, dexamethasone target genes were identified Keywords: ordered					
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Term Source Name	EFO
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Klein-Hitpass	Vassen	Fiolka	Mahlmann	Klein-Hitpass	Möröy
Person First Name	Ludger	Lothar	Katharina	Stefan	Ludger	Tarik
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Person Email	ludger.klein-hitpass@uni-essen.de					
Person Affiliation	Institut fuer Zellbiologie					
Person Phone	+49 201 723 3178					
Person Fax	+49 201 723 5904					
Person Address	Universitaetsklinikum, Institut fuer Zellbiologie, Virchowstr. 173, Essen, Germany					
Person Roles	submitter					
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Protocol Name	P-GSE2342-1					
Protocol Description	ID_REF =  <br>VALUE = 'Signal, a measure of the abundance of the transcript<br>ABS_CALL = the call in an absolute analysis that indicates whether a transcript is reliably detected (Present, P) or  not detected (Absent, A)<br>DETECTION P-VALUE = p-value that indicates the significance level of the detection (ABS_CALL) call					
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Protocol Type	bioassay_data_transformation					
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Comment[SecondaryAccession]	GSE2342					
Comment[GEOLastUpdateDate]	2011-07-26
Comment[AEExperimentType]	transcription profiling by array					
Comment[GEOReleaseDate]	2006-02-25
Comment[ArrayExpressSubmissionDate]	2005-02-25
SDRF File	E-GEOD-2342.sdrf.txt