Investigation Title	Transcription profiling time series of HOXA5 induction in human Hs578T cell line	
Comment[Submitted Name]	HOXA5 transcriptional targets	
Experimental Design	time_series_design	individual_genetic_characteristics_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo		mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-08-25	
Comment[SecondaryAccession]	GSE2241	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-GEOD-2241	
Comment[MAGETAB TimeStamp_Version]	2010-08-04 15:01:01 Last Changed Rev: 13058	
Experimental Factor Name	Time	
Experimental Factor Type	time	
Experimental Factor Term Source REF	
Person Last Name	Chen	
Person First Name	Hexin	
Person Mid Initials	
Person Email	hchen15@jhmi.edu	
Person Phone	
Person Fax	
Person Address	Johns Hopkins University, Baltimore, 21231-1000, USA	
Person Affiliation	Johns Hopkins University	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
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Date of Experiment	
Public Release Date	2007-08-25	
PubMed ID	15757903	
Publication DOI	15757903	
Publication Author List	Hexin Chen, Ethel Rubin, Huiping Zhang, Seung Chung, Charles C Jie, Elizabeth Garrett, Shyam Biswal, Saraswati Sukumar	
Publication Title	Identification of transcriptional targets of HOXA5.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	HOXA5 inducible cell line Hs578T was established and cultured as described previously (MCB 2004. 24:924-935). 2×106 cells were seeded onto a 10-cm cell culture dish at 24h before induction. Two batches of RNA from HOXA5-inducible cells (0 h, 6 h and 9 h) were purified for repeating the microarray hybridization experiments once using Affymatrix Chips.Total RNA was extracted using TRIzol reagent (Gibco BRL, Life Technologies, Grand Island, NY) and purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).	
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Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-2241.sdrf.txt	
Term Source Name	The MGED Ontology	ArrayExpress	mo	EFO	The MGED Ontology	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version