Comment[ArrayExpressAccession]	E-GEOD-14488
Public Release Date	2009-01-22		
Investigation Title	Drosophila melanogaster S2-GMR cells, second biological replicate		
Comment[Submitted Name]	Drosophila melanogaster S2-GMR cells, second biological replicate
Experiment Description	High-throughput sequencing of Drosophila melanogaster small RNAs from S2 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH  For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and  http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2 cells obtained from Gerald Rubin's lab. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.		
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Term Source Name	EFO
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Term Source File	http://www.ebi.ac.uk/efo/efo.owl		
Person Last Name	Robine	Lai	
Person First Name	Nicolas	Eric	
Person Mid Initials		C	
Person Email	robinen@mskcc.org		
Person Affiliation	Memorial Sloan Kettering Cancer Center		
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Person Address	Developmental Biology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 252, New York, NY, USA		
Person Roles	submitter		
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Protocol Name	P-GSE14488-1	P-GSE14488-2	P-GSE14488-3
Protocol Description	SEQUENCE = <br>COUNT = number of times small RNA was sequenced	total RNA from S2R+ cells, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH	raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt
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Protocol Type	bioassay_data_transformation	nucleic_acid_extraction	feature_extraction
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Publication Title	Dicing of viral replication intermediates during silencing of latent Drosophila viruses.		
Publication Author List	Flynt A, Liu N, Martin R, Lai EC		
PubMed ID	19251644		
Publication DOI	10.1073/pnas.0813412106		
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Comment[SecondaryAccession]	GSE14488	SRP000606	
Comment[GEOLastUpdateDate]	2012-03-20
Comment[AEExperimentType]	RNA-seq of non coding RNA		
Comment[GEOReleaseDate]	2009-01-22
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR013618-SRR013618		
Comment[ArrayExpressSubmissionDate]	2009-01-21
SDRF File	E-GEOD-14488.sdrf.txt