Investigation Title	Transcription profiling of human normal primary trophoblasts from placenta	
Comment[Submitted Name]	primary trophoblast study	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[ArrayExpressReleaseDate]	2008-06-11	
Comment[SecondaryAccession]	GSE1302	
Comment[AEMIAMESCORE]	3	
Comment[ArrayExpressAccession]	E-GEOD-1302	
Comment[MAGETAB TimeStamp_Version]	2011-01-22 00:01:09 Last Changed Rev: 14857	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Mecham	
Person First Name	Brig	
Person Mid Initials	
Person Email	bmecham@rics.bwh.harvard.edu	
Person Phone	
Person Fax	
Person Address	Harvard Medical School, 20 Shattuck St, Boston, 02115, USA	
Person Affiliation	Harvard Medical School	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-11	
PubMed ID	15161964	
Publication DOI	15161964	
Publication Author List	Brigham H Mecham, Daniel Z Wetmore, Zoltan Szallasi, Yoel Sadovsky, Isaac Kohane, Thomas J Mariani	
Publication Title	Increased measurement accuracy for sequence-verified microarray probes.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	Primary cultures of human trophoblasts were established from normal, term placenta. Cells were treated five hours after plating. This sample was treated with 1 uM GW7845. RNA was extracted after 48 hours of treatment using Tri-reagent. Each sample was pooled from three individual placentas prior to target generation. Targets were produced using standard Affymetrix procedures from 30ug of total RNA.	
Protocol Name	
Protocol Type	
Protocol Description	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-1302.sdrf.txt	
Term Source Name	mo	The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version