Investigation Title	Transcription profiling of human endothelial cells after 40 h and 24 h exposure to BK virus
Comment[Submitted Name]	bkv-24h-40h
Experimental Design	time_series_design	compound_treatment_design
Comment[AEExperimentType]	transcription profiling by array
Experimental Design Term Source REF	mo	mo	EFO
Comment[ArrayExpressReleaseDate]	2006-02-09
Comment[AEMIAMESCORE]	1
Comment[ArrayExpressAccession]	E-BASE-3
Comment[MAGETAB TimeStamp_Version]	2010-07-29 14:32:49 Last Changed Rev: 13058
Experimental Factor Name	Duration of Exposure	bkv
Experimental Factor Type	time	compound_treatment_design
Experimental Factor Term Source REF
Person Last Name	Grinde	unknown
Person First Name	Bjørn	unknown
Person Mid Initials
Person Email	bjorn.grinde@fhi.no
Person Phone	22042420
Person Fax
Person Address		"Nasjonalt folkehelseinstitutt   Pb 4404 Nydalen, 0403 Oslo, Norway"
Person Affiliation	Norwegian Institute of Public Health
Person Roles	submitter
Person Roles Term Source REF
Quality Control Type	biological_replicate	spike_quality_control	peer_review_quality_control
Quality Control Term Source REF
Replicate Type
Replicate Term Source REF
Normalization Type
Normalization Term Source REF
Date of Experiment
Public Release Date	2006-02-09
PubMed ID
Publication DOI
Publication Author List	Ellertsen LK, Hetland G, Johnson E, Grinde B
Publication Title	Effect of a medicinal extract from Agaricus blazei Murill on gene expression in human monocytes.
Publication Status
Publication Status Term Source REF
Experiment Description	Investigate the effect of 40 h and 24 h exposure to BK virus on human endothelial cells.
Protocol Name	microarray.no:PROT:P-89	microarray.no:PROT:P-31	microarray.no:PROT:P-90	microarray.no:PROT:P-fs6	microarray.no:PROT:P-fs16
Protocol Type	nucleic_acid_extraction	labeling	hybridization
Protocol Description	Agilent Total RNA Isolation Mini Kit used according to manufacturer's recommendations.	Aminoallyl labelling protocol  	"Automated hybridization for 12 h at 45o C with the ChipMap80 kit (including ChipHybe80 buffer) on the Ventana Discovery system (Ventana Medical systems, Tucson, USA)."
Protocol Parameters
Protocol Hardware
Protocol Software				Gene Pix	GenePix
Protocol Contact
Protocol Term Source REF
SDRF File	E-BASE-3.sdrf.txt
Term Source Name	ncbitax		ArrayExpress	mo	EFO
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html		http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/
Term Source Version