Investigation Title	Transcription profiling of doxorubicin sensitive and resistant human myeloma cell lines after treatment with doxorubicin to explore the role of the STAT1 signaling pathway in drug and radiation resistance	
Comment[Submitted Name]	050928_8226S_DOX	
Experimental Design	compound_treatment_design	co-expression_design	in_vitro_design	transcription profiling by array	
Experimental Design Term Source REF	mo	mo		EFO	
Comment[ArrayExpressReleaseDate]	2007-01-10	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-BASE-2	
Comment[MAGETAB TimeStamp_Version]	2011-06-28 11:31:43 Last Changed Rev: 14857	
Experimental Factor Name	compound	phenotype	
Experimental Factor Type	compound_treatment_design	phenotype	
Experimental Factor Term Source REF	
Person Last Name	Goransson	
Person First Name	Hanna	
Person Mid Initials	
Person Email	Hanna.Goransson@genpat.uu.se	
Person Phone	
Person Fax	
Person Address	University of Uppsala, Uppsala, Sweden	
Person Affiliation	University of Uppsala, Uppsala, Sweden	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	dye_swap_design:replicate_design	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-01-10	
PubMed ID	
Publication DOI	
Publication Author List	Fryknas M, Dhar S, Oberg F, Rickardson L, Rydaker M, Goransson H, Gustafsson M, Pettersson U, Nygren P, Larsson R, Isaksson A	
Publication Title	STAT1 signaling is associated with acquired crossresistance to doxorubicin and radiation in myeloma cell lines.	
Publication Status	
Publication Status Term Source REF	
Experiment Description	The myeloma cell line RPMI 8226/S and its doxorubicin resistant subline 8226/Dox40 were used as models to explore the potential importance of the STAT1 signaling pathway in drug and radiation resistance. The 40-fold doxorubicin resistant subline 8226/Dox40 was found to be crossresistant to single doses of 4 and 8 Gy of radiation. A genome-wide mRNA expression study comparing the 8226/Dox40 cell line to its parental line was performed to identify the underlying molecular mechanisms. Seventeen of the top 50 overexpressed genes have previously been implicated in the STAT1 signaling pathway. STAT1 was over expressed both at the mRNA and protein level. Moreover, analyses of nuclear extracts showed higher abundance of phosphorylated STAT1 (Tyr 701) in the resistant subline. Preexposure of the crossresistant cells to the STAT1 inhibiting drug fludarabine reduced expression of overexpressed genes and enhanced the effects of both doxorubicin and radiation. These results show that resistance to doxorubicin and radiation is associated with increased STAT1 signaling and can be modulated by fludarabine. The data support further development of therapies combining fludarabine and radiation.	
Protocol Name	P-BASE-Trizol	P-TABM-946	P-TABM-945	P-TABM-944	
Protocol Type		labeling	hybridization	feature_extraction	
Protocol Description	Trizol	Genisphere labelling protocol	Genesphere hybridization protocol	Genepix pro scanning protocol	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF		mo	
SDRF File	E-BASE-2.sdrf.txt	
Term Source Name	ncbitax	nci_meta	The MGED Ontology	ArrayExpress	mo	EFO	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://ncimeta.nci.nih.gov/indexMetaphrase.html	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version