E-WMIT-9 - Chromatin immunoprecipitation analysis of human hepatocyte regulatory circuitry
Submitted on 9 May 2006, released on 9 May 2006, last updated on 4 June 2014
We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Auto-regulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.
ChIP-chip by array, binding site identification
Core transcriptional regulatory circuitry in human hepatocytes. Odom, D.T.; Dowel, R.B.; Jacobsen, E.S.; Nekludova, L.; Rolfe, P.A.; Danford, T.W.; Gifford, D.K.; Fraenkel, E.; Bell, G.I.; Young, R.A. Mol Syst Biol (2006)