E-UMCU-21 - Microarray-based comparison of RSC, Condensin and Cohesin
Released on 31 December 2006, last updated on 8 November 2011
Total mRNA was isolated from parallel cultures of two Matalpha clones of each of the 7 temperature sensitive strains: STH1ts, SMC2ts, SMC3ts, STH1ts SMC2ts, STH1ts SMC3ts, SMC2ts SMC3ts, and STH1ts SMC2ts SMC3ts. The two biological replicates were processed in parallel and one was conjugated to Cy3, the other one to Cy5 dye. A competitive Cy3-Cy5 hybridization was then performed against aliquots of a pool of mRNA obtained from several clones of the congenic wild type strain. This experimental strategy was chosen to permit horizontal comparisons across the seven mutant strains.
transcription profiling by array, genetic modification