E-TIGR-18 - Transcription profiling of heat shocked Methanococcus jannaschii]

Status
Released on 1 January 2005, last updated on 2 May 2014
Organism
Methanococcus jannaschii
Samples (5)
Array (1)
Protocols (4)
Description
These experiments were designed to study the effect of heat shock and cold shock on expression profiles of a hyperthermophilic archaeon after a twenty-minute temperature shock. When cells entered the middle of the exponential growth phase at the optimal growth temperature of 85 C, they were transferred from an 85 C water bath to a 95 C water bath or a 65 C water bath. Two biological replicates were obtained under both heat shock and cold shock conditions. For each biological replicate, we performed a total of six technical replicates consisting of three pairs of flip-dye experiments. As for the reference sample, a pooled population of cDNA from three biological replicates consisting of cells growing in the mid-exponential phase at 85 C was used. All hybridizations were performed against this reference
Experiment types
transcription profiling by array, growth condition
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TIGR-18.idf.txt
Sample and data relationshipE-TIGR-18.sdrf.txt
Raw data (1)E-TIGR-18.raw.1.zip
Array designA-TIGR-16.adf.txt
Links