E-TIGR-128 - Transcription profiling of wild type G. sulfurreducens DL1 strain and mutant DLCN16 (delta-rpoS::Km) using fumarate as an electron acceptor
Released on 1 January 2005, last updated on 2 May 2014
Wild type G. sulfurreducens DL1 strain (see Caccavo, F., Jr., D. J. Lonergan, D. R. Lovley, M. Davis, J. F. Stolz, and M. J. McInerney. 1994. Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl Environ Microbiol 60:3752-9. see also Coppi, M. V., C. Leang, S. J. Sandler, and D. R. Lovley. 2001. Development of a genetic system for Geobacter sulfurreducens. Appl Environ Microbiol 67:3180-7.) and DLCN16 mutant (.rpoS::Km) (see Nuñez, C., L. Adams, S. Childers, and D. R. Lovley. 2004. The RpoS sigma factor in the dissimilatory Fe(III)-reducing bacterium Geobacter sulfurreducens. J Bacteriol 186:5543-6.) were grown under anaerobic conditions at 30 °C in continuous culture with a 200 ml working volume as previously described (see Esteve-Nunez, A., M. Rothermich, M. Sharma, and D. Lovley. 2005. Growth of Geobacter sulfurreducens under nutrient-limiting conditions in continuous culture. Environ Microbiol 7:641-8.). Cells were cultured at a growth rate of 0.05 h-1, steady-state cell growth was obtained after 5 volume refills and was confirmed by a constant cell density and concentrations of fumarate and succinate. Acetate (5.5 mM) was the electron donor and the limiting substrate. The electron acceptor was fumarate (30mM). Three biological replicates of control and treatment cells were obtained to produce hybridizations for this experiment.
transcription profiling by array, dye swap
DNA Microarray and Proteomic Analyses of the RpoS Regulon in Geobacter sulfurreducens. Cinthia Núñez, Abraham Esteve-Núñez, Carol Giometti, Sandra Tollaksen, Tripti Khare, Winston Lin, Derek R. Lovley, and Barbara A. Methé. J Bacteriol 188(8):2792-2800 (2006), Europe PMC 16585740