E-TIGR-10 - Transcription profiling of mouse strains responsive/unresponsive to asbestos backcrossed to either C57B or 129
Released on 14 February 2007, last updated on 4 June 2014
Asbestos disease model: Occupational lung disease comprises a wide variety of disorders caused by the inhalation or ingestion of dust particles or noxious chemical. These disorders include pneumoconiosis, asbestos-related pleural and others. Although asbestos is carcinogenic to humans, the mechanism by which it induces cancer is unknown. The study from Brody AR has shown that lung fibroblasts are activated to proliferate and produce connective tissue during the development of lung fibrosis. The 129 mouse strain does not develop asbestos-induced fibrogenesis, whereas several other inbred strains rapidly respond to inhaled fibers. David Schwartz from Duke University has bred C57B6 (high responders) to 129 (low responder) and backcrossed the F1 mice to either C57B or 129. RNA has been collected from lungs of both parental and progeny mice and we are useing microarray to generate the gene express profile. The purpose for this study is to find out the genes (either up-regulated or down-regulated) involve in the inhalation of asbestos and provide a guideline to the further study of human inhalation disease.
transcription profiling by array, compound treatment, reference, strain or line