E-TABM-997 - Transcription profiling by array of mouse mammary epithelial stem cell tumours.

Status
Submitted on 20 May 2010, released on 9 September 2010, last updated on 2 May 2014
Organism
Mus musculus
Samples (9)
Array (1)
Protocols (6)
Description
The aim of the experiment was to analyse gene expression profiles in Brca1 tumours arising from different mammary epithelial cell populations use a Cre-loxP based conditional knockout system. K14 promoter driving Cre expression caused Brca1 knockout in basal stem cells and thus stem cell origin tumours whereas Blg promoter driving Cre expression caused Brca1 knockout in luminal progenitor cells and thus progenitor origin tumours. Individual arrays were carried out on labelled cDNA made from RNA isolated from mouse mammary tumours. Only cDNA passing Almac diagnostics QC criteria were hybridised to arrays. Only arrays passing QC criteria after hybrisiation were subsequently analysed.
Experiment types
transcription profiling by array, cell type comparison, co-expression, in vivo
Contact
Citation
BRCA1 Basal-like Breast Cancers Originate from Luminal Epithelial Progenitors and Not from Basal Stem Cells.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-TABM-997.idf.txt
Sample and data relationshipE-TABM-997.sdrf.txt
Raw data (1)E-TABM-997.raw.1.zip
Processed data (1)E-TABM-997.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-TABM-997.eSet.r
Links