E-TABM-89 - Transcription profiling by array of mouse embryonic stem cells after treatment with cisplatin
Released on 27 April 2006, last updated on 29 October 2015
To gain insight in the kinetics and interplay of the predominant transcriptional responses of DNA damage signalling pathways in undifferentiated cells, mouse embryonic stem cells were exposed to cisplatin at four different time points (2, 4, 8 and 24 hr) and concentrations (1, 2, 5 and 10 uM). RNA was isolated and subjected to genome-wide expression profiling.
transcription profiling by array, compound treatment, dose response, time series
J Peter Svensson <firstname.lastname@example.org>, Bob van de Water, G Jean Horbach, Harry Vrieling, Jacqueline J.C.M. Kruse, Jan E.M. Polman, Merei Huigsloot, Micheline Giphart-Gassler, Willem G.E.J. Schoonen
A portrait of cisplatin-induced transcriptional changes in mouse embryonic stem cells reveals a dominant p53-like response. Kruse JJ, Svensson JP, Huigsloot M, Giphart-Gassler M, Schoonen WG, Polman JE, Jean Horbach G, van de Water B, Vrieling H. , Europe PMC 17327130