E-TABM-89 - Transcription profiling by array of mouse embryonic stem cells after treatment with cisplatin
Released on 27 April 2006, last updated on 2 May 2014
To gain insight in the kinetics and interplay of the predominant transcriptional responses of DNA damage signalling pathways in undifferentiated cells, mouse embryonic stem cells were exposed to cisplatin at four different time points (2, 4, 8 and 24 hr) and concentrations (1, 2, 5 and 10 uM). RNA was isolated and subjected to genome-wide expression profiling.
transcription profiling by array, compound treatment, dose response, time series
J Peter Svensson <firstname.lastname@example.org>, Bob van de Water, G Jean Horbach, Harry Vrieling, Jacqueline J.C.M. Kruse, Jan E.M. Polman, Merei Huigsloot, Micheline Giphart-Gassler, Willem G.E.J. Schoonen
A portrait of cisplatin-induced transcriptional changes in mouse embryonic stem cells reveals a dominant p53-like response. Jacqueline J.C.M. Kruse; J. Peter Svensson; Merei Huigsloot; Micheline Giphart-Gassler; Willem G.E.J Schoonen; Jan E.M. Polman; G. Jean Horbach; Bob van de Water; Harry Vrieling. , Europe PMC 17327130