E-TABM-877 - Transcriptional profiling of mouse pancreatic beta-cells, embryonic stem cells and 10 other normal adult tissues

Status
Submitted on 26 January 2010, released on 19 March 2010, last updated on 2 May 2014
Organism
Mus musculus
Samples (13)
Array (1)
Protocols (5)
Description
To gain insights into how pancreatic beta-cells are programmed in vivo, we profiled key histone methylations (H3K4/K27me3) in embryonic stem cells, multipotent progenitors of the nascent embryonic pancreas, purified beta-cells, and 10 other adult tissues (all under normal, untreated conditions). For these cells we also purified RNA to analyze tissue specfic genome wide transcription levels in relation to histone modifications.
Experiment types
transcription profiling by array, co-expression, in vivo, organism part comparison
Contact
Citation
Derepression of Polycomb targets during pancreatic organogenesis allows insulin-producing beta-cells to adopt a neural gene activity program. van Arensbergen, Joris; García-Hurtado, Javier; Moran, Ignasi; Maestro, Miguel Angel; Xu, Xiaobo; Van de Casteele, Mark; Skoudy, Anouchka L.; Palassini, Matteo; Heimberg, Harry; Ferrer, Jorge. Genome Res 20(6):722 (2010), Europe PMC 20395405
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TABM-877.idf.txt
Sample and data relationshipE-TABM-877.sdrf.txt
Raw data (1)E-TABM-877.raw.1.zip
Processed data (1)E-TABM-877.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-TABM-877.eSet.r
Links