E-TABM-87 - Transcription profiling of human haematological neoplasias from blood samples and cell lines
Released on 31 January 2007, last updated on 2 May 2014
Validation of a custom low density microarray. Gene expression differences were analysed in the following groups of blood samples and cell lines:
Two haematological neoplasia cell lines: U937 (promonocytic lymphoma) versus Jurkat (T leukemia).
Different types of haematological neoplasias: healthy donors, B-CLL patients, myeloid leukemia patients and U937 and Jurkat cell cultures samples.
Two hematological neoplasia with different origin (lymphoid for B-CLL and myeloid for the myeloid group).
Two types of myeloid leukemias (Acute myeloid leukemia and myelodisplastic syndrome).
B-CLL patients with different clinical behaviour.
Healthy donors and B-CLL patients.
Normalization was performed using two different methods, robust quantile normalization (QRN) and variance stabilization normalization (VSN), so that a set of statistcially significant probes found using both methods could be obtained.
transcription profiling by array, disease state, transcription profiling
Transcriptional Profiling of Hematologic Malignancies with a Low-Density DNA Microarray. Patricia Álvarez, Pilar Sáenz, David Arteta, Antonio Martínez, Miguel Pocoví, Laureano Simón, Pilar Giraldo. , Europe PMC 17185367