E-TABM-87 - Transcription profiling of human haematological neoplasias from blood samples and cell lines

Status
Released on 31 January 2007, last updated on 2 May 2014
Organism
Homo sapiens
Samples (89)
Array (1)
Protocols (12)
Description
Validation of a custom low density microarray. Gene expression differences were analysed in the following groups of blood samples and cell lines:
Two haematological neoplasia cell lines: U937 (promonocytic lymphoma) versus Jurkat (T leukemia).
Different types of haematological neoplasias: healthy donors, B-CLL patients, myeloid leukemia patients and U937 and Jurkat cell cultures samples.
Two hematological neoplasia with different origin (lymphoid for B-CLL and myeloid for the myeloid group).
Two types of myeloid leukemias (Acute myeloid leukemia and myelodisplastic syndrome).
B-CLL patients with different clinical behaviour.
Healthy donors and B-CLL patients.

Normalization was performed using two different methods, robust quantile normalization (QRN) and variance stabilization normalization (VSN), so that a set of statistcially significant probes found using both methods could be obtained.
Experiment types
transcription profiling by array, disease state, transcription profiling
Contact
Citation
Transcriptional Profiling of Hematologic Malignancies with a Low-Density DNA Microarray. Patricia Álvarez, Pilar Sáenz, David Arteta, Antonio Martínez, Miguel Pocoví, Laureano Simón, Pilar Giraldo. , Europe PMC 17185367
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-TABM-87.idf.txt
Sample and data relationshipE-TABM-87.sdrf.txt
Raw data (1)E-TABM-87.raw.1.zip
Processed data (1)E-TABM-87.processed.1.zip
Array designA-MEXP-336.adf.txt
R ExpressionSetE-TABM-87.eSet.r
Links