E-TABM-556 - Chromatin immunopreciptiation of H2AZ in mouse embryonic stem cells and neural precursor cells
Submitted on 4 September 2008, released on 22 January 2009, last updated on 2 May 2014
Elucidating how chromatin organization influences gene expression patterns and ultimately cell fate is fundamental to understanding development and disease. Histone variants have emerged as key regulators of genome function by creating specialized chromatin domains. The histone variant H2AZ plays an essential, but poorly understood function during early mammalian development. Genome-wide analysis reveals that H2AZ is enriched at a large class of developmentally important genes that are known targets of Polycomb-mediated repression in embryonic stem (ES) cells. H2AZ displays a highly defined spatial patterning that is remarkably similar to the Polycomb group (PcG) protein Suz12 in ES cells, but not in differentiated cell types. By using RNA interference, we demonstrate that H2AZ is a critical regulatory component at developmental genes in ES cells and show that localization of H2AZ and PcG proteins is interdependent at target promoters. Moreover, similarly to Suz12, H2AZ is required for lineage commitment. This study reveals a connection between H2AZ and PcG proteins in ES cells and suggests that these factors functionally interact to regulate chromatin states necessary for the proper execution of developmental gene expression programs.
ChIP-chip by array, binding site identification, cell type comparison
The histone variant H2AZ is enriched at Polycomb group target genes in ES cells and is required for control of developmental gene expression programs. M. P. Creyghton, S. Markoulaki, S. S. Levine, J. Hanna, M. A. Lodato, K. Sha, R. A. Young, R. Jaenisch, L. Boyer. Cell