6 protocols
AccessionNameType
P-TABM-2889
bioassay_data_transformation
Data Processing/Normalization: Affymetrix GCOS/MAS algorithm used to generate probeset-level intensities in TXT metrics file.
GeneSpring RMA preprocessor and algorithm used to generate probeset-level intensities in RMA file.
GeneSpring GC-RMA preprocessor and algorithm to generate probeset-level intensities in GMA file.
P-TABM-2888
feature_extraction
Image Analysis Software: GCOS ; Image Analysis Algorithm: GCOS
P-TABM-2886
hybridization
Washing/Staining Procedure: FS450_0001 ; Washing/Staining Instrumentation: Fluidics Station 450
P-TABM-2885
labeling
cDNA Synthesis: Affymetrix GeneChip One-Cycle cDNA Synthesis Kit ; cDNA Cleanup: Affymetrix GeneChip Sample Cleanup Module ; cRNA Synthesis: Affymetrix GeneChip Expression 3'-Amplification Kit for IVT Labeling ; cRNA Cleanup: Affymetrix GeneChip Sample Cleanup Module ; Spike Target Element: mRNA+Target ; Spiking Control: Affymetrix standard
P-TABM-2883
specified_biomaterial_action
Cell/Tissue Separation Technique: microdissection
P-TABM-2884
nucleic_acid_extraction
Nucleic Acid Extraction Method: phenol-based method, silica columns ; Nucleic Acid Type: total_RNA ; Nucleic Acid Cleanup: Qiagen RNeasy MinElute ; Amplification Method: RNA polymerase - single round