E-TABM-531 - Transcription profiling of mice mutant for Sox8 and Sox9 has early testicular sterility

Status
Released on 3 January 2013, last updated on 2 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (6)
Description
To study a possible redundant role of mouse Sox8 and Sox9 after testis induction we generated double mutants by AMH-Cre conditional inactivation of Sox9 in a Sox8-/- background. Double mutants arrest spermatogenesis in early pubescence. We performed expression profiling to explore causes. AMH-Cre/+;Sox9flox/flox;Sox8-/- is a conditional homozygous knockout of SOX9 in a sox8-/- background, using an AMH-CRE line where the CRE recombinase is expressed under the promoter region of the human AMH gene in Sertoli cells starting at E13.5. The specific cross was between males of the genotype +/+;Sox9flox/flox;Sox8-/- and females of the genotype AMH-Cre/+;Sox9flox/flox;Sox8+/-.
Experiment types
transcription profiling by array, co-expression, development or differentiation, disease state, genetic modification, in vivo
Contact
Citation
Genome-wide identification of Sox8-, and Sox9-dependent genes during early post-natal testis development in the mouse. F. Chalmel,A. Lardenois,I. Georg, F. Barrionuevo,P. Demougin, B. Jegou, G. Scherer† and M. Primig. ANDROLOGY  (2012), Europe PMC 23315995
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-TABM-531.idf.txt
Sample and data relationshipE-TABM-531.sdrf.txt
Raw data (1)E-TABM-531.raw.1.zip
Processed data (1)E-TABM-531.processed.1.zip
Array designA-AFFY-45.adf.txt
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