E-TABM-440 - Transcription profiling of human keratinocytes after stimulation with recombinant human cytokines to study the events controlling cell migration)

Status
Released on 7 July 2008, last updated on 12 October 2011
Organism
Homo sapiens
Samples (26)
Array (1)
Protocols (4)
Description
Cellular decisions leading to a sustained cellular migration constitute a critical parameter during wound healing and metastasis. Cellular decision processes for migration involve signaling pathways as well as gene expression regulation that have been not yet studied in an integrated way. Here, heterologous cocultures combining primary human keratinocytes and genetically defined murine fibroblasts served as a model for wound healing of human skin to gain a systemic view of the underlying principles of cellular decision making towards migration. By inverse modeling of time series of gene expression data followed by experimental validation we show that (I) pulse-like activation of the proto-oncogene receptor Met by its ligand HGF triggers a responsive system state, (II) permanent, time sequential activation of the EGF-receptor is required to initiate and sustain migration, and (III), context information for enhancing, delaying or stopping migration is provided via the activity of the PKA-signaling pathway. Our study reveals a complex orchestration of events controlling cell migration.
Experiment types
transcription profiling by array, co-expression, compound treatment, in vitro, stimulus or stress, time series
Contact
Citations
Gene network dynamics controlling keratinocyte migration. Busch H, Camacho-Trullio D, Rogon Z, Breuhahn K, Angel P, Eils R, Szabowski A.
Boolean approach to signalling pathway modelling in HGF-induced keratinocyte migration. Amit Singh; Juliana M. Nascimento; Silke Kowar; Hauke Busch; Melanie Boerries. FIX:Bioinformatics 28(18):i495 (2012), Europe PMC 22962472
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-TABM-440.idf.txt
Sample and data relationshipE-TABM-440.sdrf.txt
Raw data (1)E-TABM-440.raw.1.zip
Processed data (1)E-TABM-440.processed.1.zip
Array designA-AFFY-44.adf.txt
Links