E-TABM-401 - Chromatin immunoprecipitation of mouse B3 pre-B cells and VL3 thymocytes to identify cohesin and CTCF binding sites
Released on 31 January 2008, last updated on 12 October 2011
Identification of cohesin (Rad21) and CTCF binding sites in ~2% of the mouse genome in B3 pre-B cells stably expressing FLAG-tagged Rad21 using anti-FLAG (Sigma F3165) and anti-CTCF (Upstate 07729) antibodies
ChIP-chip by array, binding site identification, in vitro, individual genetic characteristics
Cohesins functionally associate with CTCF on mammalian chromosome arms. Vania Parelho, Suzana Hadjur, Mikhail Spivakov, Marion Leleu, Stephan Sauer,Heather C Gregson, Adam Jarmuz, Claudia Canzonetta, Zoe Webster, Tatyana Nesterova, Bradley S Cobb, Amanda G Fisher and Matthias Merkenschlager.