E-TABM-401 - Chromatin immunoprecipitation of mouse B3 pre-B cells and VL3 thymocytes to identify cohesin and CTCF binding sites

Status
Released on 31 January 2008, last updated on 12 October 2011
Organism
Mus musculus
Samples (6)
Arrays (2)
Protocols (10)
Description
Identification of cohesin (Rad21) and CTCF binding sites in ~2% of the mouse genome in B3 pre-B cells stably expressing FLAG-tagged Rad21 using anti-FLAG (Sigma F3165) and anti-CTCF (Upstate 07729) antibodies
Experiment types
ChIP-chip by array, binding site identification, in vitro, individual genetic characteristics
Contact
Citation
Cohesins functionally associate with CTCF on mammalian chromosome arms. Vania Parelho, Suzana Hadjur, Mikhail Spivakov, Marion Leleu, Stephan Sauer,Heather C Gregson, Adam Jarmuz, Claudia Canzonetta, Zoe Webster, Tatyana Nesterova, Bradley S Cobb, Amanda G Fisher and Matthias Merkenschlager.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TABM-401.idf.txt
Sample and data relationshipE-TABM-401.sdrf.txt
Raw data (1)E-TABM-401.raw.1.zip
Processed data (1)E-TABM-401.processed.1.zip
Array designsA-MEXP-966.adf.txt, A-MEXP-967.adf.txt
Links