E-TABM-285 - Transcription profiling of eyes from wild type and Bma knock-out mice after different lengths of time in a 12:12-hr LD cycle or in constant darkness to identify daily rhythms in ocular gene expression
Submitted on 14 June 2007, released on 24 August 2007, last updated on 2 May 2014
To explore daily rhythms of ocular gene expression in adult mice we performed the following experiments: i) Mice were entrained to a 12:12-hr light-dark (LD) cycle for 3 weeks. Then mice were transferred to constant darkness (DD) or remained in LD and eyes were collected at four-hour intervals over a three-day period; ii) Bmal1-/- mice and wild-type littermates were entrained to a 12:12-hr LD cycle for 3 weeks and eyes were collected at four-hour intervals over a one-day period in LD.
transcription profiling by array, co-expression, genetic modification, in vivo, organism part comparison, time series
Intrinsic circadian clock of the mammalian retina: importance for retinal processing of visual information. Kai-Florian Storch, Carlos Paz, James Signorovitch, Elio Raviola, Basil Pawlyk, Tiansen Li, Charles J. Weitz. Cell 130(4):730-41 (2007)