E-TABM-217 - Poly(A) tail length profiling of mRNAs from fission yeast

Status
Submitted on 5 February 2007, released on 16 May 2007, last updated on 2 May 2014
Organism
Schizosaccharomyces pombe
Samples (2)
Array (1)
Protocols (6)
Description
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarrays using total eluate as a reference.
Experiment types
other, co-expression, translational bias
Contact
Citation
A Network of Multiple Regulatory Layers Shapes Gene Expression in Fission Yeast. Daniel H. Lackner, Traude H. Beilharz, Samuel Marguerat, Juan Mata, Stephen Watt, Falk Schubert, Thomas Preiss and J�rg B�hler. Mol Cell in review
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-TABM-217.idf.txt
Sample and data relationshipE-TABM-217.sdrf.txt
Raw data (1)E-TABM-217.raw.1.zip
Processed data (1)E-TABM-217.processed.1.zip
Array designA-MEXP-585.adf.txt
R ExpressionSetE-TABM-217.eSet.r
Links