E-TABM-160 - Transcription profiling of D.melanogaster homozygous Toll10B gain of function mutants vs. wild type embryos at developmental stages 5-7, 8-10 and 10-12 hours

Status
Submitted on 9 October 2006, released on 28 March 2007, last updated on 2 May 2014
Organism
Drosophila melanogaster
Samples (6)
Array (1)
Protocols (4)
Description
Expression profiling of Drosophila Toll10B homozygous mutant embryos (carrying a dominant active form of the Toll transmembrane receptor) in a timecourse of embryogenesis. Three one-hour timepoints were assayed (3-6 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to INDAC oligonucleotide arrays.
Experiment types
transcription profiling by array, development or differentiation, dye swap, genetic modification, time series, transcript identification
Contact
Citation
A core transcriptional network for early mesoderm development in Drosophila melanogaster. Sandmann T, Girardot C, Brehme M, Tongprasit W, Stolc V, Furlong EE. Genes Dev 21(4):436-49 (2007), Europe PMC 17322403
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-TABM-160.idf.txt
Sample and data relationshipE-TABM-160.sdrf.txt
Raw data (1)E-TABM-160.raw.1.zip
Array designA-MEXP-314.adf.txt
R ExpressionSetE-TABM-160.eSet.r
Links