E-TABM-159 - Chromatin immunoprecipitation of Twist protein during embryonic development in Drosophila
Released on 28 March 2007, last updated on 2 May 2014
Genomewide mapping of Drosophila Twist protein binding during embryonic development. Two consecutive timepoints (2-4 and 4-6 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Twist protein. Additionally, preimmune-serum was used as a control. The enriched DNA was hybridized to custom designed tiling arrays optimized for assaying all E-box motifs outside repetitive or coding regions of the Drosophila melanogaster genome (average gap size = 290 bps).
ChIP-chip by array, binding site identification, time series
A core transcriptional network for early mesoderm development in Drosophila melanogaster. Sandmann T, Girardot C, Brehme M, Tongprasit W, Stolc V, Furlong EE. Genes Dev 21(4):436-49 (2007), Europe PMC 17322403