E-TABM-1184 - Chromatin immunoprecipitation of Drosophila to identify pMad, dTcf, Doc, Pnr and Slp1 proteins binding during embryonic development

Status
Released on 3 February 2012, last updated on 2 May 2014
Organism
Drosophila melanogaster
Samples (2)
Array (1)
Protocols (17)
Description
Genomewide mapping of Drosophila melanogaster pMad, dTcf, Doc, Pnr and Slp1 protein binding during embryonic development. Two consecutive timepoints (4-6 and 6-8 hrs after egg-laying) were assayed in two independent repeats each. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to high density Affymetrix GeneChip Drosophila Tiling 1.0R array.
Experiment types
ChIP-chip by array, binding site identification,development or differentiation,reference,replicate,time series
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Citation
A transcription factor collective defines cardiac cell fate and reflects lineage history. Junion G, Spivakov M, Girardot C, Braun M, Gustafson EH, Birney E, Furlong EE. Cell 148(3):476-86 (2012), Europe PMC 22304916
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