E-TABM-108 - Chromatin immunoprecipitation of tissue-specific transcriptional regulators in mouse and human liver to measure binding at regulatory regions of homologous genes
Submitted on 20 September 2006, released on 3 October 2006, last updated on 2 May 2014
Homo sapiens, Mus musculus
To identify experimentally both conserved and evolving aspects of transcriptional regulation, we have measured the binding of key transcriptional regulators, RNA polymerase II and histone methylation in mouse and human liver at regulatory regions of homologous genes. As expected, the set of genes for which regulator binding is conserved is enriched for known liver-specific genes, conserved binding events are associated with sequence motifs in both species, and target genes show conserved gene expression. Strikingly, a significant number of homlogous genes are bound in only one species, and expression of these genes is no more conserved than expected by chance.
ChIP-chip by array, binding site identification
Tissue-specific transcriptional regulation has diverged significantly between human and mouse. Odom DT, Dowell RD, Jacobsen ES, Gordon W, Danford TW, Macisaac KD, Rolfe PA, Conboy CM, Gifford DK, Fraenkel E. Nat Genet 39(6):730-732 (2007), Europe PMC 17529977