E-TABM-100 - Transcription profiling of Bordetella pertussis from mid-log phase cultures diluted to two concentrations and grown beyond the log to stationary phase transition to determine growth phase-associated patterns of transcript abundance
Released on 31 December 2006, last updated on 2 May 2014
Bordetella bronchiseptica, Bordetella parapertussis, Bordetella pertussis
B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until three hours beyond the log to stationary phase transition.
transcription profiling by array, genetic modification, time series
Craig Cummings <email@example.com>, Mari Nakamura
Growth Phase- and Nutrient Limitation-Associated Transcript Abundance Regulation in Bordetella pertussis. Nakamura, Mari M.; Liew, Sin-Yee; Cummings, Craig A.; Brinig, Mary M.; Dieterich, Christine; Relman, David A. Infect Immun 74(10):5537 (2006), Europe PMC 16988229