E-SMDB-3919 - Transcription profiling of C. elegans lin-14 mutants

Status
Released on 5 January 2007, last updated on 4 June 2014
Organism
Caenorhabditis elegans
Samples (0)
Arrays (2)
Protocols (2)
Description
A temporal gradient of the novel nuclear protein LIN-14 specifies the timing and sequence of stage-specific developmental events in Caenorhabditis elegans. The profound effects of lin-14 mutations on worm development suggest that LIN-14 directly or indirectly regulates stage-specific gene expression. We show that LIN-14 can associate with chromatin in vivo and has in vitro DNA binding activity. A bacterially expressed C-terminal domain of LIN-14 was used to select DNA sequences that contain a putative consensus binding site from a pool of randomized double-stranded oligonucleotides. To identify candidates for genes directly regulated by lin-14, we employed DNA microarray hybridization to compare the mRNA abundance of C. elegans genes in wild-type animals to that in mutants with reduced or elevated lin-14 activity. Five of the candidate LIN-14 target genes identified by microarrays, including the insulin/insulin-like growth factor family gene ins-33, contain putative LIN-14 consensus sites in their upstream DNA sequences. Genetic analysis indicates that the developmental regulation of ins-33 mRNA involves the stage-specific repression of ins-33 transcription by LIN-14 via sequence-specific DNA binding. These results reinforce the conclusion that lin-14 encodes a novel class of transcription factor.
Experiment types
transcription profiling by array, Logical Set
Contacts
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-SMDB-3919.idf.txt
Sample and data relationshipE-SMDB-3919.sdrf.txt
Raw data (1)E-SMDB-3919.raw.1.zip
Processed data (1)E-SMDB-3919.processed.1.zip
Array designsA-SMDB-379.adf.txt, A-SMDB-79.adf.txt
Links