E-SMDB-3835 - Transcription profiling of primary skin fibrolasts from Niemann-Pick type C patients homozygous for the I1061T mutation

Status
Submitted on 2 January 2007, released on 5 January 2007, last updated on 15 May 2013
Organism
Homo sapiens
Samples (0)
Array (1)
Protocols (3)
Description
Primary skin fibroblasts from four Niemann-Pick type C patients homozygous for the I1061T mutation and four control individuals were cultured under identical conditions in DMEM containing 10% fetal bovine serum. Cells were harvested at 50-70% confluency. mRNA was isolated with the FastTrack 2.0 mRNA isolation kit according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). A reference RNA comprised of 10 cell lines was used as the control for each hybridized sample (Stratagene, La Jolla, CA). Both sample and reference RNAs were amplified using the MessageAmp II aRNA amplification kit (Ambion, Austin, TX).
Experiment types
transcription profiling by array, cell type comparison, co-expression
Contacts
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-SMDB-3835.idf.txt
Sample and data relationshipE-SMDB-3835.sdrf.txt
Raw data (1)E-SMDB-3835.raw.1.zip
Processed data (1)E-SMDB-3835.processed.1.zip
Array designA-SMDB-1087.adf.txt
Links