E-SMDB-3835 - Transcription profiling of primary skin fibrolasts from Niemann-Pick type C patients homozygous for the I1061T mutation
Submitted on 2 January 2007, released on 5 January 2007, last updated on 15 May 2013
Primary skin fibroblasts from four Niemann-Pick type C patients homozygous for the I1061T mutation and four control individuals were cultured under identical conditions in DMEM containing 10% fetal bovine serum. Cells were harvested at 50-70% confluency. mRNA was isolated with the FastTrack 2.0 mRNA isolation kit according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). A reference RNA comprised of 10 cell lines was used as the control for each hybridized sample (Stratagene, La Jolla, CA). Both sample and reference RNAs were amplified using the MessageAmp II aRNA amplification kit (Ambion, Austin, TX).
transcription profiling by array, cell type comparison, co-expression
Clues to neuro-degeneration in niemann-pick type C disease from global gene expression profiling. Reddy JV, Ganley IG, Pfeffer SR. PLoS ONE 1:e19 (2006), Europe PMC 17183645