E-SMDB-2960 - Transcription profiling of wild type vs pp65 mutant CMV infected HFFs

Status
Released on 7 November 2005, last updated on 4 June 2014
Organism
Homo sapiens
Samples (0)
Arrays (2)
Protocols (1)
Description
Polyadenylated RNA from 10^7 confluent HFs was purified by using Oligotex (QIAGEN, Valencia, Calif.) from Trizol (Invitrogen)-extracted total RNA. This RNA was prepared, reverse transcribed, labeled with Cy3-dUTP or Cy5-dUTP (Amersham, Little Chalfont, Buckinghamshire, United Kingdom) by random primed synthesis with DNA pol I Klenow (Amersham Life Science, Inc., Cleveland, Ohio), and hybridized to spotted, human cDNA microarrays as previously described. Sequence-verified human cDNA microarrays (HE and HG series, 31,000 spots; HD51 series, 17,000 spots) were produced at Stanford. Images were collected by using a GenePix 4000B microarray scanner, manually flagged to eliminate poor spots and analyzed by GenePix Pro 2.0 (Axon, Union City, Calif.).
Experiment types
transcription profiling by array, disease state
Contacts
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-SMDB-2960.idf.txt
Sample and data relationshipE-SMDB-2960.sdrf.txt
Raw data (1)E-SMDB-2960.raw.1.zip
Processed data (1)E-SMDB-2960.processed.1.zip
Array designsA-SMDB-370.adf.txt, A-SMDB-462.adf.txt
Links