E-SMDB-2880 - RNAi knock down of Hepatitis B viral gene expression in human HepG2.2.15 or PLC/PRF/5 cells

Status
Submitted on 3 November 2005, released on 3 November 2005, last updated on 27 March 2012
Organism
Homo sapiens
Samples (0)
Array (1)
Protocols (4)
Description
Gene expression profiling comparisons of HepG2.2.15 or PLC/PRF/5 cells either mock (M) transfected or transfected with 0.2 microM S2 RNA or Scrambled (SCR) siRNA were carried out in duplicate 48 hours after transfection. The experiments were carried out in duplicate (a and b). The following combinations of RNA were used on 2 slides each: 1. 2.2.15 cells: mock transfection (reference) versus S2 treatment (test) 2. 2.2.15 cells: mock transfection (reference) versus Scr treatment (test) 3. 2.2.15 cells: Scr treatment (reference) versus S2 treatment (test) 4. PLC/PRF/5 cells: mock transfection (reference) versus S2 treatment (test) 5. PLC/PRF/5 cells: mock transfection (reference) versus Scr treatment (test) 6. PLC/PRF/5 cells: Scr treatment (reference) versus S2 treatment (test)
Experiment types
RNAi profiling by array, stimulus or stress
Contacts
Citation
Genome-wide expression profiling of RNA interference of hepatitis B virus gene expression and replication. Li Y, Wasser S, Lim SG, Tan TM. Cell Mol Life Sci 61(16):2113-24 (2004-08-01), Europe PMC 15316660
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-SMDB-2880.idf.txt
Sample and data relationshipE-SMDB-2880.sdrf.txt
Raw data (1)E-SMDB-2880.raw.1.zip
Processed data (1)E-SMDB-2880.processed.1.zip
Array designA-SMDB-923.adf.txt
Links