E-SMDB-2669 - Transcription profiling of human childrens host response to malaria
Submitted on 6 July 2005, released on 6 July 2005, last updated on 27 March 2012
Subjects are children from Kilifi District Hospital, Kenya, recruited in 2001 malaria season. total RNA extracted from PaxGene tubes, total RNA and Stratagene Universal reference RNA underwent linear amplification (Message Amp, Ambion). Amplified RNA directly labelled Cy5 and Cy3 and hybridised to 'lymphochip' arrays (print run - lc36n). Scanned on Axon 4000B scanner using GenePix 4.0 software. Clinical sample ID provided in 'Disease State Description' corresponds with Sample ID in Table 1 "Clinical Information for the micro-array samples" in the associated publication.
transcription profiling by array, disease state
Genomewide Analysis of the Host Response to Malaria in Kenyan Children. Griffiths MJ , Shafi MJ , Popper SJ , Hemingway CA , Kortok MM , Wathen A , Rockett KA , Mott R , Levin M , Newton CR , Marsh K , Relman DA , Kwiatkowski DP. J Infect Dis 191:1599-1611 (2005-05-15), Europe PMC 15838786