E-SMDB-2653 - Transcription profiling of 10 individual human cell lines to identify unique genes contributed by individual cell lines to UHRR

Status
Submitted on 1 November 2005, released on 1 November 2005, last updated on 4 June 2014
Organism
Mus musculus
Samples (0)
Array (1)
Protocols (1)
Description
RNA from 11 individual mouse cell lines were reverse-transcribed to cDNA, labeled with Cy5 and cohybridized with Cy3-labeled UMRR onto 7,500-spot mouse oligo microarrays (UNC). The data was analyzed using GeneTraffic software. 300-1000 spots out of 8,000 were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics on only one microarray was determined.
Experiment types
transcription profiling by array, Expression (Type II), reference
Contacts
Citation
Universal Reference RNA as a standard for microarray experiments. Novoradovskaya N, Whitfield ML, Basehore LS, Novoradovsky A, Pesich R, Usary J, Karaca M, Wong WK, Aprelikova O, Fero M, Perou CM, Botstein D, Braman J. BMC Genomics 5(1):20 (2004), Europe PMC 15113400
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-SMDB-2653.idf.txt
Sample and data relationshipE-SMDB-2653.sdrf.txt
Raw data (1)E-SMDB-2653.raw.1.zip
Processed data (1)E-SMDB-2653.processed.1.zip
Array designA-SMDB-531.adf.txt
Links