E-SMDB-2623 - Transcription profiling of S. cervisiae wild type (BY4741) and Puf3 mutant cells grown in minimal medium supplemented with 3% glycerol

Status
Submitted on 1 November 2005, released on 1 November 2005, last updated on 27 March 2012
Organism
Saccharomyces cerevisiae
Samples (0)
Array (1)
Protocols (4)
Description
Three independent experiments: S. cervisiae wild-type (BY4741) and Puf3 mutant cells were grown in minimal medium supplemented with 3% glycerol. Cells were harvested in mid-log phase by centrifugation and total RNA was prepared by hot-phenol extraction. cDNA was prepared with oligo-dT and using a mixture of amino-allyl dUTP and dNTPs, fluorescently labeled (Cy3= wild-type, Cy5 = mutant) and hybridize on S. cerevisiae DNA microarray.
Experiment types
transcription profiling by array, Expression (Type I), unknown experiment type
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-SMDB-2623.idf.txt
Sample and data relationshipE-SMDB-2623.sdrf.txt
Raw data (1)E-SMDB-2623.raw.1.zip
Processed data (1)E-SMDB-2623.processed.1.zip
Array designA-SMDB-835.adf.txt
Links