E-SMDB-2373 - Transcription profiling of parasites grown as either tachyzoites or bradyzoites for 40 hours pulsed with 2,4-dithiouracil (DTU) for 1 hour

Status
Submitted on 7 July 2005, released on 7 July 2005, last updated on 2 May 2014
Organism
Toxoplasma gondii
Samples (0)
Array (1)
Protocols (4)
Description
Parasites grown as either tachyzoites or bradyzoites for 40 hours were pulsed with 2,4-dithiouracil (DTU) for 1 hour. Following the pulse, mRNA was extracted and either used directly in microarray experiments (Abundance Arrays) or biotinylated and purified to select DTU labeled RNAs that were then used in microarray experiments (Synthesis Arrays). Two independent tachyzoite and bradyzoite preparations were made and duplicate microarrays were performed for each sample.
Experiment types
transcription profiling by array, development or differentiation
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-SMDB-2373.idf.txt
Sample and data relationshipE-SMDB-2373.sdrf.txt
Raw data (1)E-SMDB-2373.raw.1.zip
Processed data (1)E-SMDB-2373.processed.1.zip
Array designA-SMDB-722.adf.txt
Links